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[Effects of moxibustion on the expression of cell cycle protein P 16 and retinoblastoma and c-fos in the cerebral cortex of senile mice].
Behav Brain Res. 2004 Aug 12;153(1):181-8. PMID: 21090325
Department of Acu-moxibustion, the Affiliated Ruikang Hospital of Guangxi College of Chinese Medicine, Nanning 530011, China. This email address is being protected from spambots. You need JavaScript enabled to view it.
OBJECTIVE: To observe the influence of moxibustion on the cyclin and cellular proliferin of the cerebral cortex in senile mice so as to explore its underlying mechanism in delaying aging.
METHODS: Sixty male mice were randomly and equally divided into control, model, moxibustion of "Zusanli" (ST 36) and "Xuanzhong" (GB 39, M-ST 36-GB 39), moxibustion of "Baihui" (GV 20) and "Guanyuan" (CV 4,M-GV 20-COV 4) ,and medication groups. The aging model was established by subcutaneous injection of D-galactose for 42 days. Moxibustion was applied to ST 36, GB 39, GV 20 and CV 4 separately for 3 moxa-cones, once every other day for one month. The expression of cell cycle protein P 16 and retinoblastoma (pRb), and c-fos protein in the cerebral cortex tissue of the senile mice were detected by immunohistochemistry.
RESULTS: Compared with control group, the number of P16 immunoreaction (IR) positive neurons in the cerebral cortex increased significantly in the model group (P<0.01), and those of cortical pRb and c-fos IR-positive neurons decreased considerably in model group (P<0.01). In comparison with the model group, the number of cortical P 16 IR-positive neurons reduced significantly in M-ST 36-GB 39, M-GV 20-CV 4 and medication groups (P<0.01), and those of cortical pRb and c-fos IR-positive neurons increased remarkably in M-ST 36-GB 39, M-GV 20-CV 4 and medication groups (P<0.01, P<0.05). No significant differences were found in the aforementioned 3 indexes among M-ST 36-GB 39, M-GV 20-CV 4 and medication groups (P>0.05).
CONCLUSION: Moxibustion of ST 36-GB 39 and GV 20-CV 4 can down-regulate the P 16 expression,and up-regulate pRb and c-fos protein expression in the cerebral cortex of senile mice, which possibly contributes to its effect in delaying aging.
