Cybermedlife - Therapeutic Actions Heat Therapy

Positive response of a primary leiomyosarcoma of the breast following salvage hyperthermia and pazopanib. 📎

Abstract Title: Positive response of a primary leiomyosarcoma of the breast following salvage hyperthermia and pazopanib. Abstract Source: Korean J Intern Med. 2016 Apr 15. Epub 2016 Apr 15. PMID: 27079325 Abstract Author(s): Sun Young Lee, Na-Ri Lee Article Affiliation: Sun Young Lee Abstract: No Abstract Available Article Published Date : Apr 14, 2016

Hyperthermia synergizes with chemotherapy by inhibiting PARP1-dependent DNA replication arrest. 📎

Abstract Title: Hyperthermia synergizes with chemotherapy by inhibiting PARP1-dependent DNA replication arrest. Abstract Source: Cancer Res. 2016 Mar 24. Epub 2016 Mar 24. PMID: 27013194 Abstract Author(s): Lea Schaaf, Matthias Schwab, Christoph Ulmer, Simon Heine, Thomas E Mürdter, Jens O Schmid, Georg Sauer, Walter E Aulitzky, Heiko van der Kuip Article Affiliation: Lea Schaaf Abstract: Although hyperthermia offers clinical appeal to sensitize cells to chemotherapy, this approach has been limited in terms of long-term outcome as well as economic and technical burden. Thus, a more detailed knowledge about how hyperthermia exerts its effects on chemotherapy may illuminate ways to improve the approach. Here we asked whether hyperthermia alters the response to chemotherapy-induced DNA damage and if this mechanism is involved in its sensitizing effect, in BRCA-competent models of ovarian and colon cancer. Notably, we found that hyperthermia delayed the repair of DNA damage caused by cisplatin or doxorubicin, acting upstream of different repair pathways to block histone polyADP-ribosylation (PARylation), a known effect of chemotherapy. Further, hyperthermia blocked this histone modification as efficiently as pharmacological inhibitors of polyADP-ribosyl polymerase (PARPi), producing comparable delay in DNA repair, induction of double strand breaks (DSB) and cell cytotoxicity after chemotherapy. Mechanistic investigations indicated that inhibiting PARylation by either hyperthermia or PARPi induced lethal DSB upon chemotherapy treatment, not only by reducing DNA repair but also by preventing replication fork slowings. Overall, our work reveals how PARP blockade - either by hyperthermia or small molecule inhibition - can increase chemotherapy-induced damage in BRCA-competent cells. Article Published Date : Mar 23, 2016

Antiproliferative and Proapoptotic Effects of Crocin Combined with Hyperthermia on Human Breast Cancer Cells.

Abstract Title: Antiproliferative and Proapoptotic Effects of Crocin Combined with Hyperthermia on Human Breast Cancer Cells. Abstract Source: DNA Cell Biol. 2016 Mar 22. Epub 2016 Mar 22. PMID: 27003728 Abstract Author(s): Seyedeh Elham Mostafavinia, Mohsen Khorashadizadeh, Reyhane Hoshyar Article Affiliation: Seyedeh Elham Mostafavinia Abstract: We investigated the suppressive effects of crocin alone and in combination with hyperthermia (HT) on proliferation of breast cancer cells. Cell viability, colony formation ability, and apoptosis were assessed by 3-(4,5-dimetylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT), soft agar, Hoechst 33258 staining, and percentage of lactate dehydrogenase (LDH) release methods, respectively. The mRNA levels Hsp27, Hsp70, Hsp90, Bax, and Bcl-2 were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Hsp70 and Hsp90 proteins were determined using enzyme-linked immunosorbent assay (ELISA) technique. Crocin in combination with HT significantly inhibited the proliferation of cancer cells in a dose- and time-dependent manner. There was a degree of synergism in the combined treatment. However, crocin did not show the high cytotoxic effect on normal cells. This treatment decreased colony formation of cancer cells up to 94%. Changed nuclear morphology and increased LDH indicated that crocin combined with HT has a more apoptotic effect than crocin alone. Furthermore, in treated cells Bax/Bcl-2 ratio markedly increased, whereas expression of heat-induced genes decreased. Also, the Hsp70 and Hsp90 proteins decreased in the treated cells. Our study indicated that combination of crocin and HT has strong antiproliferative and apoptotic activities against breast cancer cells. Hence, it is suggested that more studies are warranted to apply crocin as a possible, safe, and promising anticancer agent in cancer. Article Published Date : Mar 21, 2016

Targeted near infrared hyperthermia combined with immune stimulation for optimized therapeutic efficacy in thyroid cancer treatment. 📎

Abstract Title: Targeted near infrared hyperthermia combined with immune stimulation for optimized therapeutic efficacy in thyroid cancer treatment. Abstract Source: Oncotarget. 2016 Feb 9 ;7(6):6878-90. PMID: 26769848 Abstract Author(s): Le Zhou, Mengchao Zhang, Qingfeng Fu, Jingting Li, Hui Sun Article Affiliation: Le Zhou Abstract: Treatment of thyroid cancer has incurred much focus because of its high prevalency. As a new strategy treating thyroid cancer, hyperthermia takes several advantages compared with surgery or chemotherapy, including minimal invasion, low systematic toxicity and the ability to enhance the immunogenicity of cancer cells with the expression Hsp70 which serves as Toll-like receptors-4 (TLR-4 agonist). However, Hsp70 as a molecular chaperone can protect cells from heat induced apoptosis and therefore compromise the tumor killing effect of hyperthermia. In this study, to solve this problem, a combined hyperthermia therapy was employed to treat thyroid cancer. We prepared a probe with the tumor targeting agent AG to monitor thyroid tumor issue and generate heat to kill tumor cells in vivo. At the same time Quercetin (inhibitor of HSP70) and lipopolysaccharide (LPS) (agonist of TLR-4) were used for the combined hyperthermia therapy. The results showed that compared with free IR820, AG modification facilitated much enhanced cellular uptake and greatly pronounced tumor targeting ability. The combined therapy exhibited the most remarkable tumor inhibition compared with the single treatments both in vitro and in vivo. These findings verified that the new therapeutic combination could significantly improve the effect of hyperthermia and shed light on a novel clinical strategy in thyroid cancer treatment. Article Published Date : Feb 08, 2016

Quercetin induces apoptosis and enhances 5-FU therapeutic efficacy in hepatocellular carcinoma.

Abstract Title: Quercetin induces apoptosis and enhances 5-FU therapeutic efficacy in hepatocellular carcinoma. Abstract Source: Tumour Biol. 2015 Dec 1. Epub 2015 Dec 1. PMID: 26628295 Abstract Author(s): Wei Dai, Quangen Gao, Jianping Qiu, Jianmao Yuan, Guoliang Wu, Genhai Shen Article Affiliation: Wei Dai Abstract: Quercetin (Q), a flavonoid compound, which is obtained in variety of fruits, seeds, and vegetables, has been reported to possess many pharmacological properties including cancer-preventive and anticancer effects. However, studies on the anticancer effects and underlying mechanisms of Q in human hepatocellular carcinoma (HCC) are still limited. The present study is conducted to investigate the anticancer efficacy and adjuvant chemotherapy action of Q in HCC. HCC cell lines HepG2 and SMCC-7721 were treated with different concentrations of Q. The antiproliferative effects of Q were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and the apoptosis and cell cycle dynamics were assessed by flow cytometry; the expression of apoptosis-associated proteins were evaluated by Western blot and immunohistochemistry staining; the tumor growth in vivo was evaluated in a xenograft mouse model. Our results showed that Q effectively inhibited human HCC cell proliferation and induced apoptosis by upregulating the expression of Bad and Bax and downregulating the expression of Bcl-2 and Survivin in vitro. Furthermore, Q obviously inhibited the tumor growth and enhanced the 5-fluorouracil (5-FU) therapeutic efficacy in vitro and in vivo. Taken together, our findings highlight that Q effectively inhibited the growth of tumor and enhanced the sensitivity to thermotherapy, indicating Q is a potential treatment option for HCC. Article Published Date : Nov 30, 2015

Efficacy of hyperbaric oxygen therapy combined with mild hyperthermia for improving the anti-tumour effects of carboplatin.

Abstract Title: Efficacy of hyperbaric oxygen therapy combined with mild hyperthermia for improving the anti-tumour effects of carboplatin. Abstract Source: Int J Hyperthermia. 2015 ;31(6):643-8. Epub 2015 Jul 9. PMID: 26156211 Abstract Author(s): Takayuki Ohguri, Naoki Kunugita, Katsuya Yahara, Hajime Imada, Hidehiko Uemura, Nadayoshi Shinya, Gotou Youjirou, Chijiwa Takashi, Ryuji Okazaki, Akira Ootsuyama, Yukunori Korogi Article Affiliation: Takayuki Ohguri Abstract: PURPOSE: The aim of this study was to evaluate the effects of hyperbaric oxygen therapy (HBO) on the enhancement of hyperthermic chemosensitisation to carboplatin at mild temperatures in experimental tumours. METHODS: SCCVII carcinoma in C3H/He mice was used to assess tumour growth delay. The mice received intraperitoneal injections of carboplatin. For HBO treatment, the mice were exposed to HBO at 2.0 atmospheres of absolute oxygen for 60 min. For mild hyperthermia (HT), treatment at 41.5 °C for 30 min was performed. The tumour tissue pO2 levels were measured with a digital pO2 monitor during and immediately after treatment. RESULTS: The average time taken to reach a threefold relative tumour size was significantly longer after treatment with carboplatin combined with mild HT and HBO than after treatment with carboplatin and mild HT. The relative sizes of the tumours after the combined treatment were smallest when the treatment sequence was carboplatin, mild HT, and HBO. The tumour tissue pO2 values were significantly higher immediately after mild HT followed by HBO than immediately after HBO followed by mild HT. The tumour tissue pO2 levels during mild HT and HBO generally increased, although the patterns of the increases varied. CONCLUSION: The administration of HBO increased the effects of hyperthermic chemosensitisation to carboplatin at mild temperatures on experimental tumours, particularly when given in the sequence of carboplatin, mild HT, and HBO, a finding that supports previous clinical outcomes for a novel combined therapy using carboplatin plus HT and HBO. Article Published Date : Dec 31, 2014

Hyperthermia combined with 5-fluorouracil promoted apoptosis and enhanced thermotolerance in human gastric cancer cell line SGC-7901. 📎

Abstract Title: Hyperthermia combined with 5-fluorouracil promoted apoptosis and enhanced thermotolerance in human gastric cancer cell line SGC-7901. Abstract Source: Onco Targets Ther. 2015 ;8:1265-70. Epub 2015 May 27. PMID: 26064061 Abstract Author(s): Tao Liu, Yan-Wei Ye, A-Li Zhu, Zhen Yang, Yang Fu, Chong-Qing Wei, Qi Liu, Chun-Lin Zhao, Guo-Jun Wang, Xie-Fu Zhang Article Affiliation: Tao Liu Abstract: This study was designed to investigate the proliferation inhibition and apoptosis-promoting effect under hyperthermia and chemotherapy treatment, at cellular level. Human gastric cancer cell line SGC-7901 was cultivated with 5-fluorouracil at different temperatures. Cell proliferation and apoptosis were determined, and expression of Bcl-2 and HSP70 was measured at different treatments. Cell survival rates and inhibition rates in chemotherapy group, thermotherapy group, and thermo-chemotherapy group were drastically lower than the control group (P<0.05). For tumor cells in the thermo-chemotherapy group, survival rates and inhibition rates at three different temperatures were all significantly lower than those in chemotherapy group and thermotherapy group (P<0.05). 5-Fluorouracil induced apoptosis of SGC-7901 cells with a strong temperature dependence, which increased gradually with increase in temperature. At 37°C and 43°C there were significant differences between the thermotherapy group and chemotherapy group and between the thermo-chemotherapy group and thermotherapy group (P<0.01). The expression of Bcl-2 was downregulated and HSP70 was upregulated, with increase in temperature in all groups. Cell apoptosis was not significant at 46°C (P>0.05), which was probably due to thermotolerance caused by HSP70 accumulation. These results suggested that hyperthermia combined with 5-fluorouracil had a synergistic effect in promoting apoptosis and enhancing thermotolerance in gastric cancer cell line SGC-7901. Article Published Date : Dec 31, 2014

Inhibitory effects of mild hyperthermia plus docetaxel therapy on ER(+/-) breast cancer cells and action mechanisms.

Abstract Title: Inhibitory effects of mild hyperthermia plus docetaxel therapy on ER(+/-) breast cancer cells and action mechanisms. Abstract Source: J Huazhong Univ Sci Technolog Med Sci. 2013 Dec ;33(6):870-6. Epub 2013 Dec 13. PMID: 24337851 Abstract Author(s): Feng Lv, Yang Yu, Bin Zhang, Dong Liang, Zhao-ming Li, Wei You Article Affiliation: Feng Lv Abstract: The purpose of this study was to verify that a combination of mild hyperthermia and docetaxel chemotherapy produces synergistic antitumor effects and to explore the action mechanisms of this treatment approach. The effects of docetaxel on the proliferation of cells from the estrogen receptor (ER)-positive human breast cancer cell line MCF-7 and the ER-negative human breast cancer cell line MDA-MB-453 were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and effective experimental concentrations of docetaxel were determined. The effects of mild hyperthermia plus docetaxel therapy on apoptosis rate in the MCF-7 and MDA-MB-453 human breast cancer cell lines were analyzed by using flow cytometry with Annexin-V fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. The effects of these combined treatments on cell cycle progression in the MCF-7 and MDA-MB-453 human breast cancer cell lines were examined by using flow cytometry. The effects of these combined treatments on the expression of apoptosis-related proteins and proteins in the mitogen-activated protein kinase (MAPK) pathways were analyzed by using Western blotting. The effects of these combined treatments on the expression of the heat shock protein 70 (HSP70) and the multi-drug resistance (MDR) gene product P-glycoprotein (Pgp) were examined by using Western blotting. The results showed that the half-maximal inhibitory concentration (IC50) of docetaxel for MCF-7 and MDA-MB-453 cells was 19.57±1.12 and 21.64±2.31 μmol/L respectively. Mild hyperthermia with docetaxel therapy could increase apoptosis rate in the MCF-7 and MDA-MB-453 cells. Apoptosis rate in MCF-7 and MDA-MB-453 cells was increased from (23.66±3.59)% and (18.51±3.17)% in docetaxel treatment group to (47.12±6.73)% and(55.16±7.42)% in mild hyperthermia plus docetaxel group, indicating that the mild hyperthermia and docetaxel therapeutic approaches exhibited significant synergistic antitumor effects. Treatments of mild hyperthermia plus docetaxel induced G2/M cell cycle arrest in the MCF-7 and MDA-MB-453 cells. Western blotting demonstrated that proteins in the MAPK pathway were expressed at higher levels in docetaxel-treated cells following mild hypothermia than those in cells treated with docetaxel alone. As compared with blank control group, cells from the mild hyperthermia plus docetaxel group exhibitedsignificantly decreased B-cell lymphoma 2 (Bcl-2) protein expression but slightly increased Bcl-2-associated X protein (Bax) expression. Western blotting results revealed that HSP70 and Pgp expression levels were significantly increased following mild hypothermia. It was concluded that treatments of mild hyperthermia plus docetaxel inhibited the proliferation of human breast cancer cells, promoted apoptosis of breast cancer cells, and produced synergistic antitumor effects. Article Published Date : Nov 30, 2013

Synergistic effect of thermotherapy in combination with chemotherapy on lung tumor A549 cells growth through activation of c-Jun N-terminal kinase and inhibition of heat shock protein70 expression

Abstract Title: [Synergistic effect of thermotherapy in combination with chemotherapy on lung tumor A549 cells growth through activation of c-Jun N-terminal kinase and inhibition of heat shock protein70 expression]. Abstract Source: Wei Sheng Yan Jiu. 2008 Sep ;37(5):529-32. PMID: 19069644 Abstract Author(s): Shan Gao, Lin Wang, Weidong Wu, Fang Zhou Article Affiliation: Shan Gao Abstract: OBJECTIVE: To study the synergistic effect of thermotherapy and chemotherapy with chemotherapy on lung tumor cell growth in order to explore its underlying mechanisms. METHODS: A549 cells were treated by combination of 43 degrees C and 50 microg/L Paclitaxel, and 43 degrees C or 50 microg/L Paclitaxel alone. MTU assay and Wound-healing assay were used to measure the survival and the invasive capacity. Phosphorylation of JNK and expression of HSP70 were determined with Western Blotting. Cells without treatment were used as controls. RESULTS: In comparison with cells treated with 43 degrees C and 50 microg/L Paclitaxel alone, or with combination of 43 degrees C and 50 microg/L Paclitaxel and SP600125, the proliferation rate of cells subjected to combination treatment of 43 degrees C and 50 microg/L Paclitaxel decreased significantly (P>0.05). There were no significant differences in cell proliferation between untreated cells and the cells treated with 43 degrees C heat, 50 microg/L Paclitaxel and SP600125 (P>0.05). The invasive capacities of the cells treated combination of 43 degrees C heat and 50 microg/L Paclitaxel were markedly reduced in comparison to other treatment groups. Also phosphorylations of JNK were significantly elevated in comparison to untreated cells or the cells only underwent chemotherapy (P<0.05). The expression levels of HSP70 on thermotherapy in combination with chemotherapy were more lower than those in thermotherapy group (P<0.05). CONCLUSION: Thermotherapy in combination with chemotherapy showed more strong inhibitory effect than those of thermotherapy or chemotherapy alone on lung tumor cell growth. The resultant phosphorylation of JNK and inhibition of HSP70 expression could be responsible for this effect. Article Published Date : Aug 31, 2008

Effects of deep and superficial heating in the management of frozen shoulder. 📎

Abstract Title: Effects of deep and superficial heating in the management of frozen shoulder. Abstract Source: J Rehabil Med. 2008 Feb;40(2):145-50. PMID: 18509580 Abstract Author(s): May S F Leung, Gladys L Y Cheing Article Affiliation: Physiotherapy Department, Our Lady of Maryknoll Hospital, Hong Kong. Abstract: OBJECTIVES: To determine whether the addition of deep or superficial heating to stretching produces better clinical outcomes than stretching alone in the management of frozen shoulder. DESIGN: A single-blinded, randomized controlled study. SUBJECTS: Thirty subjects suffering from the stiffness phase of frozen shoulder. METHODS: Subjects were randomly allocated to receive: (i) deep heating plus stretching; (ii) superficial heating plus stretching; or (iii) stretching alone. Both heating groups received the respective treatments 3 times per week for 4 weeks. All groups received a standard set of shoulder stretching exercises. The American Shoulder and Elbow Surgeons assessment form was recorded at the baseline, sessions 6 and 12, and at the 4-week follow-up session. RESULTS: A significant improvement was seen in all groups in all outcome measures except for that of shoulder flexion range. The improvement in the shoulder score index and in the range of motion was significantly better in the deep heating group than in the superficial heating group. CONCLUSION: The addition of deep heating to stretching exercises produced a greater improvement in pain relief, and resulted in better performance in the activities of daily living and in range of motion than did superficial heating. Article Published Date : Feb 01, 2008
Therapeutic Actions Heat Therapy

NCBI pubmed

Heat Shock Gene Inactivation and Protein Aggregation with Links to Chronic Diseases.

Related Articles Heat Shock Gene Inactivation and Protein Aggregation with Links to Chronic Diseases. Diseases. 2018 May 18;6(2): Authors: Martins IJ Abstract The heat shock response involved in protein misfolding is linked to the formation of toxic immunogenic proteins with heat shock proteins (HSP) as regulators of amyloid beta aggregation. The defective amyloid beta trafficking between different intracellular compartments is now relevant to HSPs and autoimmunity. Overnutrition, temperature dysregulation, and stress repress the heat shock gene Sirtuin 1 with the induction of HSP regulated amyloid beta aggregation involved in the autoimmune response. Defective circadian rhythm alterations are connected to inactivation of the peripheral sink amyloid beta clearance pathway and related to insulin resistance, protein aggregation, and autoimmune disease in non-alcoholic fatty liver disease (NAFLD) and various neurodegenerative diseases such as Alzheimer's disease. Nutritional therapy is critical to prevent immunosenescence, and plasma Sirtuin 1 levels should be determined to reverse, stabilize, and prevent protein aggregation with relevance to mitochondrial apoptosis and programmed cell death in chronic diseases. PMID: 29783682 [PubMed]

Application of Albumin-embedded Magnetic Nanoheaters for Release of Etoposide in Integrated Chemotherapy and Hyperthermia of U87-MG Glioma Cells.

Related Articles Application of Albumin-embedded Magnetic Nanoheaters for Release of Etoposide in Integrated Chemotherapy and Hyperthermia of U87-MG Glioma Cells. Anticancer Res. 2018 05;38(5):2683-2690 Authors: Babincová M, Vrbovská H, Sourivong P, Babinec P, Durdík Š Abstract BACKGROUND/AIM: Malignant gliomas remain refractory to several therapeutic approaches and the requirement for novel treatment modalities is critical to combat this disease. Etoposide is a topoisomerase-II inhibitor, which promotes DNA damage and apoptosis of cancer cells. In this study, we prepared albumin with embedded magnetic nanoparticles and etoposide for in vitro evaluation of combined hyperthermia and chemotherapy. MATERIAL AND METHODS: Magnetic nanoparticles were prepared by a modified co-precipitation method in the presence of human serum albumin and etoposide. A cellular proliferation assay was used to determine the effects of these nanostructures on the viability of U87 glioma cells in an alternating magnetic field. RESULTS: The in vitro experiments showed that cell viability decreased to 59.4% after heat treatment alone and to 53.8% on that with free etoposide, while combined treatment resulted in 7.8% cell viability. CONCLUSION: Integrating hyperthermia and chemotherapy using albumin co-embedded magnetic nanoheaters and etoposide may represent a promising therapeutic option for glioblastoma. PMID: 29715088 [PubMed - indexed for MEDLINE]

Assessing the Links Between Anthropometrics Data and Akabane Test Results.

Related Articles Assessing the Links Between Anthropometrics Data and Akabane Test Results. J Acupunct Meridian Stud. 2018 Feb;11(1):31-38 Authors: Muzhikov V, Vershinina E, Belenky V, Muzhikov R Abstract According to popular belief, metabolic disorders and imbalances are one of the main factors contributing to various human illnesses. Early diagnosis of these disorders is one of the main methods for preventing serious diseases. The goal of this study was to assess the correlations between main physical indicators and the activity of certain acupuncture channels using the thermal Akabane test based on ancient Chinese diagnostic methods. This test measures the pain thresholds' temperature sensitivity when a point source of heat is applied to the "entrance-exit" points of each channel. The skin temperature sensitivity in our bodies is a basic reactive system; it is as significant as such important indicators as body temperature and provides a very clear representation of functional and psychophysiological profiles. On the basis of our statistical study, we revealed reliable correspondence between the activity of certain acupuncture channels and main anthropometric and biometric data. PMID: 29482799 [PubMed - indexed for MEDLINE]

The cryo-electron microscopy structure of huntingtin.

Related Articles The cryo-electron microscopy structure of huntingtin. Nature. 2018 03 01;555(7694):117-120 Authors: Guo Q, Bin Huang, Cheng J, Seefelder M, Engler T, Pfeifer G, Oeckl P, Otto M, Moser F, Maurer M, Pautsch A, Baumeister W, Fernández-Busnadiego R, Kochanek S Abstract Huntingtin (HTT) is a large (348 kDa) protein that is essential for embryonic development and is involved in diverse cellular activities such as vesicular transport, endocytosis, autophagy and the regulation of transcription. Although an integrative understanding of the biological functions of HTT is lacking, the large number of identified HTT interactors suggests that it serves as a protein-protein interaction hub. Furthermore, Huntington's disease is caused by a mutation in the HTT gene, resulting in a pathogenic expansion of a polyglutamine repeat at the amino terminus of HTT. However, only limited structural information regarding HTT is currently available. Here we use cryo-electron microscopy to determine the structure of full-length human HTT in a complex with HTT-associated protein 40 (HAP40; encoded by three F8A genes in humans) to an overall resolution of 4 Å. HTT is largely α-helical and consists of three major domains. The amino- and carboxy-terminal domains contain multiple HEAT (huntingtin, elongation factor 3, protein phosphatase 2A and lipid kinase TOR) repeats arranged in a solenoid fashion. These domains are connected by a smaller bridge domain containing different types of tandem repeats. HAP40 is also largely α-helical and has a tetratricopeptide repeat-like organization. HAP40 binds in a cleft and contacts the three HTT domains by hydrophobic and electrostatic interactions, thereby stabilizing the conformation of HTT. These data rationalize previous biochemical results and pave the way for improved understanding of the diverse cellular functions of HTT. PMID: 29466333 [PubMed - indexed for MEDLINE]

[Effects of extractionfrom raspberry on hippocampus proteomics of mice suffered from ovariectomized-induced AD].

Related Articles [Effects of extractionfrom raspberry on hippocampus proteomics of mice suffered from ovariectomized-induced AD]. Zhongguo Zhong Yao Za Zhi. 2016 Aug;41(15):2895-2900 Authors: Hou M, Fu YJ, Liu C, Wei YF, Chen YF, Xie YH, Huang LP Abstract This paper was aimed to investigate the impact of the extraction from raspberry on the Alzheimer disease model protein expression. According to weight, the ovariectomized mice were randomly divided into shame operation group, model group, estrogen positive control group(0.1 g•L⁻¹) and ethyl acetate extraction part control group(in dose of 18 g•kg⁻¹). Each mouse in positive control group was subcutaneous injected of estradiol with 0.2 mL every two days. Raspberry effective parts group were given 0.01 mL•g⁻¹ raspberry ethylacetate extracts, model group and control group were given 0.01 mL•g⁻¹ saline once a day. The drug administration lasted for 32 days. Proteins from mice's hippocampus were extracted, then Nanol-ESI liquid-mass spectrometry system was used for detection and ProteinDiscovery software was used for identification to qualitative analysis different groups of hippocampal proteins by using the software of SIEVE. The results showed that model group compared with the mice of ethyl acetate extraction part control group have 66 differentially expressed proteins including heat shock protein, microtubule protein, protein involved in energy metabolism and protein of brain protection related proteins associated with AD. Those differences protein may be the target that Raspberry prevention and treatment of AD. PMID: 28914035 [PubMed - indexed for MEDLINE]

Cold Acclimation Does Not Alter Physiological or Perceptual Responses During Subsequent Exercise in the Heat.

Related Articles Cold Acclimation Does Not Alter Physiological or Perceptual Responses During Subsequent Exercise in the Heat. Mil Med. 2017 Sep;182(9):e1958-e1964 Authors: Jones DM, Rostomily KA, Pautz CM, Ito DW, Bailey SP, Roelands B, Buono MJ, Meeusen R Abstract INTRODUCTION: Warfighters often train and conduct operations in cold environments. Specifically, military trainees and divers that are repeatedly exposed to cold water may experience inadvertent cold acclimatization, which results in body heat retention. These same warfighters can quickly switch between environments (cold to hot or hot to cold) given the nature of their work. This may present a risk of early onset of hyperthermia when cold-acclimatized warfighters are subsequently exposed to physiological insults that increase body temperature, such as exercise and heat stress. However, there is currently no evidence that suggests this is the case. The purpose of this work, therefore, is to determine what impact, if any, repeated immersion in cold water has on subsequent exercise in the heat. MATERIALS AND METHODS: Twelve healthy subjects (values in mean ± SD: age, 25.6 ± 5.2 years; height, 174.0 ± 8.9 cm; weight, 75.6 ± 13.1 kg) voluntarily provided written informed consent in accordance with the San Diego State University Institutional Review Board. They first completed 120 minutes of moderate treadmill walking in 40°C and 40% relative humidity. During this trial, subjects' physiological and perceptual responses were recorded. Twenty-four hours later, subjects began a cold acclimation protocol, which consisted of seven, 90-minute immersions in cold water (10°C, water level to chest). Each immersion was also separated by 24 hours. Subjects then repeated a subsequent trial of exercise in the heat 24 hours after the final immersion of the cold acclimation protocol. RESULTS: Results from cold acclimation revealed no change in core temperature, a decrease in skin temperature, and attenuated shivering and lactate responses, which supports a successful insulative-hypothermic cold acclimation response. This type of cold acclimation response primarily results in heat retention with associated energy conservation. Findings for heat trials (pre-cold acclimation and post-cold acclimation) revealed no differences between trials for all measurements, suggesting that cold acclimation did not influence physiological or perceptual responses during exercise in the heat. CONCLUSION: Our findings indicate that military divers or trainees that are frequently exposed to cold water, and hence have the ability to experience cold acclimatization, will likely not be at greater risk of increased thermal strain when subsequently exposed to physical activity in hot environments. In this study, no physiological or perceptual differences were observed between trials before and after cold acclimation, suggesting that cold acclimation does not present a greater hyperthermia risk during subsequent exercise in the heat. PMID: 28885962 [PubMed - indexed for MEDLINE]

Effectiveness of Ice-Sheet Cooling Following Exertional Hyperthermia.

Related Articles Effectiveness of Ice-Sheet Cooling Following Exertional Hyperthermia. Mil Med. 2017 Sep;182(9):e1951-e1957 Authors: Butts CL, Spisla DL, Adams JD, Smith CR, Paulsen KM, Caldwell AR, Ganio MS, McDermott BP Abstract BACKGROUND: The procedure of wrapping a heat casualty in ice-water soaked bed sheets to reduce core temperature has received little investigation, despite the practice and recommendation for its use in some military settings. Thus, the purpose of this study was to investigate the cooling efficacy of ice-sheet cooling (ISC) following exertional hyperthermia. METHODS: 13 (11 males, 2 females) participants (age = 23 ± 3 years, height = 176.5 ± 10.3 cm, mass = 78.6 ± 15.3 kg, body fat = 19.6 ± 8.6%, and body surface area = 1.95 ± 0.22 m2) volunteered to complete 2 randomized, crossover design trials on an outdoor recreation field (34.4 ± 1.4°C, 54.4 ± 4.1% relative humidity). Each trial consisted of exercise (self-paced 400-m warm-up, 1,609-m run, and 100-m sprints) followed by 15 minutes of either lying supine in the shade with no treatment (control [CON]) or being treated with ice-water soaked sheets wrapped around their body (ISC). Physiological (rectal temperature [Tre], heart rate, mean-weighted skin temperature) and perceptual measures (thermal sensation, rating of perceived exertion) were assessed after each exercise protocol, every 3 minutes during treatment, and every 5 minutes during recovery. FINDINGS: By design, there were no differences during exercise between ISC and CON for Tre (p = 0.16), skin temperature (p = 0.52), heart rate (p = 0.62), thermal sensation (p = 0.89), or rating of perceived exertion (p = 0.99). There were greater decreases in Tre at 3 (ISC 0.33 ± 0.26°C vs. CON 0.03 ± 0.30°C, p = 0.01) and 6 minutes (ISC 0.47 ± 0.27°C vs. CON 0.30 ± 0.19°C, p = 0.05) of treatment; however, the overall rate of cooling was not different between trials (CON 0.05 ± 0.02°C/min vs. ISC 0.06 ± 0.02°C/min, p = 0.72). Skin temperature (Tsk) was significantly reduced from 3 minutes (ISC 34.4 ± 1.7°C vs. CON 36.6 ± 0.5°C, p = 0.007) through 15 minutes (ISC 32.4 ± 1.5 vs. CON 36.1 ± 0.4°C, p < 0.001) of treatment. There was a trend for lower heart rate with ISC (p = 0.051). Thermal sensation was reduced from 3 minutes of treatment (ISC 3.5 ± 0.9 vs. CON 4.5 ± 0.6, p = 0.002) through 15 minutes (ISC 2.8 ± 1.0 vs. CON 3.9 ± 0.4, p = 0.005). DISCUSSION: ISC does not provide effective reduction in Tre following exertional hyperthermia compared to no treatment. However, perceptual benefits may warrant the use of ISC in settings where rapid reductions in core temperature are not a concern (i.e., recovery from exercise). Thus, clinicians should continue to utilize validated techniques (i.e., cold-water immersion) for the treatment of exertional heat illnesses. PMID: 28885961 [PubMed - indexed for MEDLINE]

Ultrasound-Induced Bubble Clusters in Tissue-Mimicking Agar Phantoms.

Related Articles Ultrasound-Induced Bubble Clusters in Tissue-Mimicking Agar Phantoms. Ultrasound Med Biol. 2017 Oct;43(10):2318-2328 Authors: Movahed P, Kreider W, Maxwell AD, Dunmire B, Freund JB Abstract Therapeutic ultrasound can drive bubble activity that damages soft tissues. To study the potential mechanisms of such injury, transparent agar tissue-mimicking phantoms were subjected to multiple pressure wave bursts of the kind being considered specifically for burst wave lithotripsy. A high-speed camera recorded bubble activity during each pulse. Various agar concentrations were used to alter the phantom's mechanical properties, especially its stiffness, which was varied by a factor of 3.5. However, the maximum observed bubble radius was insensitive to stiffness. During 1000 wave bursts of a candidate burst wave lithotripsy treatment, bubbles appeared continuously in a region that expanded slowly, primarily toward the transducer. Denser bubble clouds are formed at higher pulse repetition frequency. The specific observations are used to inform the incorporation of damage mechanisms into cavitation models for soft materials. PMID: 28739379 [PubMed - indexed for MEDLINE]

Variation of High-Intensity Therapeutic Ultrasound (HITU) Pressure Field Characterization: Effects of Hydrophone Choice, Nonlinearity, Spatial Averaging and Complex Deconvolution.

Related Articles Variation of High-Intensity Therapeutic Ultrasound (HITU) Pressure Field Characterization: Effects of Hydrophone Choice, Nonlinearity, Spatial Averaging and Complex Deconvolution. Ultrasound Med Biol. 2017 Oct;43(10):2329-2342 Authors: Liu Y, Wear KA, Harris GR Abstract Reliable acoustic characterization is fundamental for patient safety and clinical efficacy during high-intensity therapeutic ultrasound (HITU) treatment. Technical challenges, such as measurement variation and signal analysis, still exist for HITU exposimetry using ultrasound hydrophones. In this work, four hydrophones were compared for pressure measurement: a robust needle hydrophone, a small polyvinylidene fluoride capsule hydrophone and two fiberoptic hydrophones. The focal waveform and beam distribution of a single-element HITU transducer (1.05 MHz and 3.3 MHz) were evaluated. Complex deconvolution between the hydrophone voltage signal and frequency-dependent complex sensitivity was performed to obtain pressure waveforms. Compressional pressure (p+), rarefactional pressure (p-) and focal beam distribution were compared up to 10.6/-6.0 MPa (p+/p-) (1.05 MHz) and 20.65/-7.20 MPa (3.3 MHz). The effects of spatial averaging, local non-linear distortion, complex deconvolution and hydrophone damage thresholds were investigated. This study showed a variation of no better than 10%-15% among hydrophones during HITU pressure characterization. PMID: 28735734 [PubMed - indexed for MEDLINE]

Effect of Frequency and Focal Spacing on Transcranial Histotripsy Clot Liquefaction, Using Electronic Focal Steering.

Related Articles Effect of Frequency and Focal Spacing on Transcranial Histotripsy Clot Liquefaction, Using Electronic Focal Steering. Ultrasound Med Biol. 2017 Oct;43(10):2302-2317 Authors: Gerhardson T, Sukovich JR, Pandey AS, Hall TL, Cain CA, Xu Z Abstract This in vitro study investigated the effects of ultrasound frequency and focal spacing on blood clot liquefaction via transcranial histotripsy. Histotripsy pulses were delivered using two 256-element hemispherical transducers of different frequency (250 and 500 kHz) with 30-cm aperture diameters. A 4-cm diameter spherical volume of in vitro blood clot was treated through 3 excised human skullcaps by electronically steering the focus with frequency proportional focal spacing: λ/2, 2 λ/3 and λ with 50 pulses per location. The pulse repetition frequency across the volume was 200 Hz, corresponding to a duty cycle of 0.08% (250 kHz) and 0.04% (500 kHz) for each focal location. Skull heating during treatment was monitored. Liquefied clot was drained via catheter and syringe in the range of 6-59 mL in 0.9-42.4 min. The fastest rate was 16.6 mL/min. The best parameter combination was λ spacing at 500 kHz, which produced large liquefaction through 3 skullcaps (23.1 ± 4.0, 37.1 ± 16.9 and 25.4 ± 16.9 mL) with the fast rates (3.2 ± 0.6, 5.1 ± 2.3 and 3.5 ± 0.4 mL/min). The temperature rise through the 3 skullcaps remained below 4°C. PMID: 28716432 [PubMed - indexed for MEDLINE]

Sonodynamic Therapy Based on Combined Use of Low Dose Administration of Epirubicin-Incorporating Drug Delivery System and Focused Ultrasound.

Related Articles Sonodynamic Therapy Based on Combined Use of Low Dose Administration of Epirubicin-Incorporating Drug Delivery System and Focused Ultrasound. Ultrasound Med Biol. 2017 Oct;43(10):2295-2301 Authors: Maeda M, Muragaki Y, Okamoto J, Yoshizawa S, Abe N, Nakamoto H, Ishii H, Kawabata K, Umemura S, Nishiyama N, Kataoka K, Iseki H Abstract Sonodynamic therapy (SDT) is currently considered as one of the promising minimally invasive treatment options for solid cancers. SDT is based on the combined use of a sonosensitizer drug and high-intensity focused ultrasound (HIFU) to produce cytotoxic reactive oxygen species (ROS) in and around neoplastic cells. Anthracycline drugs, including epirubicin (EPI), have been well known as effective sonosensitizers after interaction with focused ultrasound. Recently a new anticancer drug delivery system (DDS), NC-6300, has been developed that comprises EPI through an acid-labile hydrazone bond. In previous in vivo studies, NC-6300 showed basic drug safety and an excellent concentration property of EPI, and recently has been tested in clinical trials. For realizing minimally invasive cancer treatment, the present study demonstrated the effectiveness and feasibility of DDS-based SDT, which combined a small dose of NC-6300 and low energy of HIFU in mouse models of colon cancer and pancreatic cancer. PMID: 28705555 [PubMed - indexed for MEDLINE]

Morbidity and mortality of elderly patients following cytoreductive surgery and Hyperthermic Intraperitoneal Chemotherapy (HIPEC).

Related Articles Morbidity and mortality of elderly patients following cytoreductive surgery and Hyperthermic Intraperitoneal Chemotherapy (HIPEC). Asia Pac J Clin Oncol. 2018 Apr;14(2):e193-e202 Authors: Wong EYT, Tan GHC, Chia CSL, Kumar M, Soo KC, Teo MCC Abstract AIM: Cytoreductive surgery (CRS) and Hyperthermic Intraperitoneal Chemotherapy (HIPEC) is known to improve survival in selected patients with peritoneal metastasis. However, there is limited data supporting the role of CRS and HIPEC in elderly patients (≥65 years old). METHODS: A retrospective review of a prospectively maintained database of patients who underwent CRS-HIPEC between April 2001 and July 2015 from a single institution was performed. Patients were divided into two groups non-elderly (<65 years old), and elderly (≥65 years old). Clinico- pathological parameters, morbidity and overall (OS) and disease-free survival (DFS) of the patients were compared. RESULTS: A total of 177 patients (median age 52, range 9-74) underwent CRS-HIPEC with curative intent. There were 159 non-elderly patients and 18 elderly patients. Median PCI scores were 12 (0-39) for the non- elderly patients and 11 (1-29) for the elderly patients (p=0.77). High-grade complications occurred in 39 non-elderly patients (24.5%) and 8 elderly patients (44.4%) (p=0.79), while 58 non-elderly patients (38.7%) and 7 elderly patients (41.2%) stayed in ICU for more than 1 day (p=0.69). There was no difference in the 30-day mortality between the two groups (0% vs. 0%, p=1). After a median follow-up of 16 months for all patients, there was no difference in 5-years OS (51.0% vs. 59.6%, p=0.88) and 5-years DFS (23.3% vs. 53.3%, p=0.60) between non-elderly and elderly patients. CONCLUSIONS: Surgical outcomes after CRS-HIPEC do not differ significantly between non-elderly and elderly patients. Hence, age should not be a contraindication in selecting patients for CRS and HIPEC. PMID: 28695617 [PubMed - indexed for MEDLINE]

Treatment of peritoneal carcinomatosis with hyperthermic intraperitoneal chemotherapy in colorectal cancer.

Related Articles Treatment of peritoneal carcinomatosis with hyperthermic intraperitoneal chemotherapy in colorectal cancer. ANZ J Surg. 2017 Sep;87(9):665-670 Authors: Behrenbruch C, Hollande F, Thomson B, Michael M, Warrier SK, Lynch C, Heriot A Abstract The peritoneum is the second most common site of metastasis after the liver and the only site of metastatic disease in approximately 25% of patients with colorectal cancer (CRC). In the past, peritoneal carcinomatosis in CRC was thought to be equivalent to distant metastasis; however, the transcoelomic spread of malignant cells is an acknowledged alternative pathway. Metastasectomy with curative intent is well accepted in patients with liver metastasis in CRC despite the paucity of randomized trials. Therefore, there is rationale for local treatment with peritonectomy to eliminate macroscopic disease, followed by hyperthermic intraperitoneal chemotherapy to destroy any residual free tumour cells within the peritoneal cavity. The aim of this paper is to summarize the current evidence for cytoreduction and hyperthermic intraperitoneal chemotherapy in the treatment of peritoneal carcinomatosis in CRC. PMID: 28664645 [PubMed - indexed for MEDLINE]

Study of efficacy of esthetic High-Intensity Focused Ultrasound system on Iranian skin for reducing the laxity and wrinkles of aging.

Related Articles Study of efficacy of esthetic High-Intensity Focused Ultrasound system on Iranian skin for reducing the laxity and wrinkles of aging. J Cosmet Dermatol. 2017 Sep;16(3):336-341 Authors: Saket P, Shobeihi S, Mehrdadi S Abstract BACKGROUND: Ultrasound is a mechanical wave that transmitted in a straight line and it can be focused at frequencies of 1-7 MHz with a high degree of precision. An important aspect of HIFU is that damaging focal point is located several millimeters below the surface of the skin and tissue above and below the focal point remains unaffected. Due to absorption of this energy, temperature of tissue rises to more than 60°C and this stimulates the cells for collagen production and tissue rejuvenation. OBJECTIVE: To evaluate the clinical efficacy and safety of High-Intensity Focused Ultrasound on Skin Laxity and wrinkles. MATERIAL AND METHOD: The study involved 22 women aged 35-62 with skin type II-IV. HIFU device with three transducers was employed. Various focal depths with different energies were used in accordance with skin thickness. Improvement for seven regions of face and whole face were evaluated by two dermatologists and patients 3 months after treatment. RESULTS: All of the patients completed the study. Three months after treatment, there was a highly statistical improvement in skin laxity for all of the patients. Also, the average percentage of improvement by doctor's opinion and patients was 58% and 60%, respectively. CONCLUSION: The experimental data obtained from dermatologists and patients showed that HIFU appears to be a safe and effective modality for skin laxity treatment. PMID: 28150435 [PubMed - indexed for MEDLINE]

Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells.

Related Articles Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. Sci Rep. 2016 07 22;6:30314 Authors: Cheng YC, Huang CJ, Lee YJ, Tien LT, Ku WC, Chien R, Lee FK, Chien CC Abstract This study presents human placenta-derived multipotent cells (PDMCs) as a source from which functional glutamatergic neurons can be derived. We found that the small heat-shock protein 27 (HSP27) was downregulated during the neuronal differentiation process. The in vivo temporal and spatial profiles of HSP27 expression were determined and showed inverted distributions with neuronal proteins during mouse embryonic development. Overexpression of HSP27 in stem cells led to the arrest of neuronal differentiation; however, the knockdown of HSP27 yielded a substantially enhanced ability of PDMCs to differentiate into neurons. These neurons formed synaptic networks and showed positive staining for multiple neuronal markers. Additionally, cellular phenomena including the absence of apoptosis and rare proliferation in HSP27-silenced PDMCs, combined with molecular events such as cleaved caspase-3 and the loss of stemness with cleaved Nanog, indicated that HSP27 is located upstream of neuronal differentiation and constrains that process. Furthermore, the induced neurons showed increasing intracellular calcium concentrations upon glutamate treatment. These differentiated cells co-expressed the N-methyl-D-aspartate receptor, vesicular glutamate transporter, and synaptosomal-associated protein 25 but did not show expression of tyrosine hydroxylase, choline acetyltransferase or glutamate decarboxylase 67. Therefore, we concluded that HSP27-silenced PDMCs differentiated into neurons possessing the characteristics of functional glutamatergic neurons. PMID: 27444754 [PubMed - indexed for MEDLINE]

Quantitative Study of Elasticity of Rabbit VX2 Liver Tumor with Alternated Cooling and Heating Treatment based on ARFI Ultrasound Imaging Technique.

Related Articles Quantitative Study of Elasticity of Rabbit VX2 Liver Tumor with Alternated Cooling and Heating Treatment based on ARFI Ultrasound Imaging Technique. Sci Rep. 2016 07 06;6:29303 Authors: Sun D, Wei C, Shen E, Ying T, Hu B Abstract Acoustic radiation force impulse (ARFI) ultrasound imaging technique is used to quantitatively evaluate the elasticity of rabbit VX2 liver tumor with alternated cooling and heating treatment (ACHT). ACHT was performed on fifteen VX2 liver tumor models established in fifteen male New Zealand white rabbits with open tumor plant. ARFI was performed on day 0, 1, 7 and 14 after ACHT and shear wave velocity (SWV) in ARFI was recorded to evaluate the elasticity of the treated area. The SWV value of the lesion on day 0, 1, 7 and 14 was 2.33 ± 0.19 m/s, 3.09 ± 0.40 m/s, 2.64 ± 0.37 m/s and 2.26 ± 0.24 m/s, respectively, indicating the treated areas get stiffer on day 1 and then get softer gradually by day. All the difference between adjacent time points was statistically significant. The SWV value of different parts on day 7 approved that the hardness of the treated area is heterogenous: the treated area in the center >the peripheral strip-shaped area >normal liver tissues, consistent with pathological changes. Meanwhile, ARFI combined with conventional US imaging can qualitatively and quantitatively exam the healing process of rabbit VX2 liver tumor after ACHT, and corresponds well to the pathological results. PMID: 27381362 [PubMed - indexed for MEDLINE]