Cybermedlife - Therapeutic Actions Laser Treatment - Low-Level

Contribution of photodynamic therapy in wound healing: A systematic review.

Abstract Title: Contribution of photodynamic therapy in wound healing: A systematic review. Abstract Source: Photodiagnosis Photodyn Ther. 2018 Mar ;21:294-305. Epub 2017 Dec 28. PMID: 29289704 Abstract Author(s): Vanessa Nesi-Reis, Daniele Stéfanie Sara Lopes Lera-Nonose, Jully Oyama, Marielle Priscila Paula Silva-Lalucci, Izabel Galhardo Demarchi, Sandra Mara Alessi Aristides, Jorge Juarez Vieira Teixeira, Thaís Gomes Verzignassi Silveira, Maria Valdrinez Campana Lonardoni Article Affiliation: Vanessa Nesi-Reis Abstract: OBJECTIVE: We researched articles that used photodynamic therapy (PDT) for skin wound healing in humans. METHODS: The systematic review was conducted through scientific articles that investigated the action of PDT on wound healing in humans, published from July 2005 to March 2017, in the data bases PubMed and LILACS. RESULTS: The main types of wound described in selected articles in this review were chronic ulcer and non-melanoma skin cancer. For accomplishing the PDT, second generation of photosensitizing agents with laser or light emitting diode were used. The studies demonstrated that PDT contribute in several ways to the wound healing process: leading to cellular death; reducing or increasing inflammation; stimulating fibroblasts proliferation and, consequently, of collagen and elastin; raising transforming growth factor beta and metalloproteinases. Based on this, PDT provided good results in wound healing process, acting in several steps and accelerating tissue repair. CONCLUSIONS: PDT improved healing in many wound models in humans, revealing itself as a promising therapeutic modality for stimulating wound healing and remodelling. Article Published Date : Feb 28, 2018

Optimal management of orthodontic pain. 📎

Abstract Title: Optimal management of orthodontic pain. Abstract Source: J Pain Res. 2018 ;11:589-598. Epub 2018 Mar 16. PMID: 29588616 Abstract Author(s): Francielle Topolski, Alexandre Moro, Gisele Maria Correr, Sasha Cristina Schimim Article Affiliation: Francielle Topolski Abstract: Pain is an undesirable side effect of orthodontic tooth movement, which causes many patients to give up orthodontic treatment or avoid it altogether. The aim of this study was to investigate, through an analysis of the scientific literature, the best method for managing orthodontic pain. The methodological aspects involved careful definition of keywords and diligent search in databases of scientific articles published in the English language, without any restriction of publication date. We recovered 1281 articles. After the filtering and classification of these articles, 56 randomized clinical trials were selected. Of these, 19 evaluated the effects of different types of drugs for the control of orthodontic pain, 16 evaluated the effects of low-level laser therapy on orthodontic pain, and 21 evaluated other methods of pain control. Drugs reported as effective in orthodontic pain control included ibuprofen, paracetamol, naproxen sodium, aspirin, etoricoxib, meloxicam, piroxicam, and tenoxicam. Most studies report favorable outcomes in terms of alleviation of orthodontic pain with the use of low-level laser therapy. Nevertheless, we noticed that there is no consensus, both for the drug and for laser therapy, on the doses and clinical protocols most appropriate for orthodontic pain management. Alternative methods for orthodontic pain control can also broaden the clinician's range of options in the search for better patient care. Article Published Date : Dec 31, 2017

Laser therapy for mens infertility. Part 2. Systematic review of clinical trials

Abstract Title: [Laser therapy for mens infertility. Part 2. Systematic review of clinical trials]. Abstract Source: Urologiia. 2017 Dec(6):164-171. PMID: 29376616 Abstract Author(s): O I Apolikhin, S V Moskvin Article Affiliation: O I Apolikhin Abstract: Male infertility is a multifactorial syndrome associated with a wide range of pathological conditions affecting both the sexual and other body systems: endocrine, nervous, blood, and immune. Laser therapy is a form of modern physiotherapy that applies low-intensity laser irradiation (LILI) in various parts of the body. It is widely used in all areas of modern medicine due to its high efficiency, ease of use, the absence of contraindications and side effects. The authors reviewed the results of 171 studies investigating laser therapy, of which 93 were experimental (20 in Russian and 73 international publications), four clinical and experimental (all in Russian), 74 clinical (73 in Russian and 1 in English).Along with domestic literature, publications in Russian from Belarus, Georgia, Kyrgyzstan, Uzbekistan, and Ukraine were also reviewed. The review suggests that laser therapy should be used as much as possible in the management of male infertility not just due to high effectiveness, but also because of the lack of alternatives. For local application, optimal laser irradiation regimens are pulsed LILI of red (635 nm) and infrared (904 nm) spectra. For intravenous laser blood irradiation, the authors recommend alternating a continuous LILI with a wavelength of 635 nm (red spectrum) and 365 nm (ultraviolet spectrum). Article Published Date : Nov 30, 2017

Anti-inflammatory effects of low-level laser therapy on human periodontal ligament cells: in vitro study. 📎

Abstract Title: Anti-inflammatory effects of low-level laser therapy on human periodontal ligament cells: in vitro study. Abstract Source: Lasers Med Sci. 2017 Nov 7. Epub 2017 Nov 7. PMID: 29116611 Abstract Author(s): Ji-Hua Lee, Min-Hsuan Chiang, Ping-Ho Chen, Mei-Ling Ho, Huey-Er Lee, Yan-Hsiung Wang Article Affiliation: Ji-Hua Lee Abstract: Periodontal disease is a chronic inflammatory disease that is commonly treated with surgical and nonsurgical techniques. However, both approaches have limitations. Low-level laser therapy (LLLT) has been widely applied in reducing inflammatory reactions, and research indicates that LLLT induces an anti-inflammatory effect that may enhance periodontal disease therapy. The purpose of this study was to investigate the anti-inflammatory effect of LLLT on human periodontal ligament cells (hPDLCs) in an inflammatory environment and aimed to determine the possible mechanism of action. Cells were cultured and treated with or without lipopolysaccharide (LPS) from Porphryromonas gingivalis or Escherichia coli, followed by irradiation with a gallium-aluminum-arsenide (GaAlAs) laser (660 nm) at an energy density of 8 J/cm2. Quantitative real-time polymerase chain reactions were used to assess the expression of pro-inflammatory genes, including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-8. The dual-luciferase reporter assay was used to examine nuclear factor-κB (NF-κB) transcriptional activity. An enzyme-linked immunosorbent assay was used to monitor the concentration of intracellular cyclic adenosine monophosphate (cAMP). Both LPS treatments significantly induced the mRNA expression of pro-inflammatory cytokines. However, LLLT inhibited the LPS-induced pro-inflammatory cytokine expression and elevated intracellular levels of cAMP. The LLLT inhibitory effect may function by downregulating NF-κB transcriptional activity and by increasing the intracellular levels of cAMP. LLLT might inhibit LPS-induced inflammation in hPDLCs through cAMP/NF-κB regulation. These results should be further studied to improve periodontal therapy. Article Published Date : Nov 06, 2017

Effects of photobiomodulation on experimental models of peripheral nerve injury.

Abstract Title: Effects of photobiomodulation on experimental models of peripheral nerve injury. Abstract Source: Lasers Med Sci. 2017 Oct 23. Epub 2017 Oct 23. PMID: 29063472 Abstract Author(s): L Andreo, C B Soldera, B G Ribeiro, P R V de Matos, S K Bussadori, K P S Fernandes, R A Mesquita-Ferrari Article Affiliation: L Andreo Abstract: Phototherapy has demonstrated positive effects in the treatment of peripheral nerve injury, but there is a need to investigate the dosimetric parameters. Thus, the aim of the present study was to conduct a literature review on the effects of photobiomodulation with the use of low-level laser therapy (LLLT) on the treatment of peripheral nerve injury in experimental models. The databases of PubMed/MEDLINE, SCOPUS, and SPIE Digital Library were searched for articles on the use of LLLT in experimental models of peripheral nerve injury published in English between January 2007 and March 2016. The laser parameter variability was wavelength (632.8 to 980 nm), power (10 to 190 mW), and total energy (0.15 to 90 J) in pulsed or continuous wave and single or multiple points. Eighteen original articles demonstrating the effects of LLLT on the acceleration of functional recovery, morphological aspects as well as the modulation of the expression inflammatory cytokines, and growth factors were selected. LLLT is a viable phototherapeutic modality for the treatment of peripheral nerve injury, demonstrating positive effects on the neuromuscular repair process using either red or infrared light. The majority of studies used a power of up to 50 mW andtotal energy of up to 15 J administered to multiple points. The determination of these parameters is important to the standardization of a LLLT protocol to enhance the regeneration process following a peripheral nerve injury. Article Published Date : Oct 22, 2017

Treating cognitive impairment with transcranial low level laser therapy.

Abstract Title: Treating cognitive impairment with transcranial low level laser therapy. Abstract Source: J Photochem Photobiol B. 2017 Mar ;168:149-155. Epub 2017 Feb 13. PMID: 28219828 Abstract Author(s): Jack C de la Torre Article Affiliation: Jack C de la Torre Abstract: This report examines the potential of low level laser therapy (LLLT) to alter brain cell function and neurometabolic pathways using red or near infrared (NIR) wavelengths transcranially for the prevention and treatment of cognitive impairment. Although laser therapy on human tissue has been used for a number of medical conditions since the late 1960s, it is only recently that several clinical studies have shown its value in raising neurometabolic energy levels that can improve cerebral hemodynamics and cognitive abilities in humans. The rationale for this approach, as indicated in this report, is supported by growing evidence that neurodegenerative damage and cognitive impairment during advanced aging is accelerated or triggered by a neuronal energy crisis generated by brain hypoperfusion. We have previously proposed that chronic brain hypoperfusion in the elderly can worsen in the presence of one or more vascular risk factors, including hypertension, cardiac disease, atherosclerosis and diabetes type 2. Although many unanswered questions remain, boosting neurometabolic activity through non-invasive transcranial laser biostimulation of neuronal mitochondria may be a valuable tool in preventing or delaying age-related cognitive decline that can lead to dementia, including its two major subtypes, Alzheimer's and vascular dementia. The technology to achieve significant improvement of cognitive dysfunction using LLLT or variations of this technique is moving fast and may signal a new chapter in the treatment and prevention of neurocognitive disorders. Article Published Date : Feb 28, 2017

The effects of low level laser therapy on both HIV-1 infected and uninfected TZM-bl cells.

Abstract Title: The effects of low level laser therapy on both HIV-1 infected and uninfected TZM-bl cells. Abstract Source: J Biophotonics. 2017 Jan 27. Epub 2017 Jan 27. PMID: 28128530 Abstract Author(s): Masixole Yvonne Lugongolo, Sello Lebohang Manoto, Saturnin Ombinda-Lemboumba, Malik Maaza, Patience Mthunzi-Kufa Article Affiliation: Masixole Yvonne Lugongolo Abstract: Human immunodeficiency virus (HIV-1) infection remains a major health problem despite the use of highly active antiretroviral therapy (HAART), which has greatly reduced mortality rates. Due to the unavailability of an effective vaccine and treatment that would completely eradicate the virus in infected individuals, the quest for new therapies continues. Low level laser therapy (LLLT) involves the exposure of cells to low levels of red or infrared light. LLLT has been widely used in different medical conditions, but not in HIV-1 infection. This study aimed to determine the effects of LLLT on HIV-1 infected and uninfected TZM-bl cells. Both infected and uninfected cells were irradiated at a wavelength of 660 nm with different fluences from 2 J/cm(2) to 10 J/cm(2) . Changes in cellular responses were assessed using cell morphology, viability, proliferation, cytotoxicity and luciferase activity assays. Upon data analysis, uninfected irradiated cells showed no changes in cell morphology, viability, proliferation and cytotoxicity, while the infected irradiated cells did. In addition, laser irradiation reduced luciferase activity in infected cells. Finally, laser irradiation had no inhibitory effect in uninfected cells, whereas it induced cell damage in a dose dependent manner in infected cells. Article Published Date : Jan 26, 2017

Use of low level laser therapy to control neuropathic pain: A systematic review.

Abstract Title: Use of low level laser therapy to control neuropathic pain: A systematic review. Abstract Source: J Photochem Photobiol B. 2016 Nov ;164:36-42. Epub 2016 Aug 31. PMID: 27639607 Abstract Author(s): Ana Laura Martins de Andrade, Paulo Ségio Bossini, Nivaldo Antônio Parizotto Article Affiliation: Ana Laura Martins de Andrade Abstract: Neuropathic pain can be defined as pain initiated or caused by a primary lesion or dysfunction in the central or peripheral nervous system. The low level laser therapy (LLLT) has gained great prominence as a treatment in this type of pain; however, the application parameters are still controversial in the literature. This study aimed to review the literature on the use of LLLT in neuropathic pain with the goal of establishing a"therapeutic window"for the effective use of this treatment. We analyzed 14 articles, 10 in experimental animals and 4 in humans. The results are presented in three tables, the first being for comparison of the studies' application parameters, the second showing the average and median parameters experimental studies and third showing the clinical studies embodiment. The experimental studies revealed better results for LLLT and infrared laser powers above 70mW. Clinical studies are inconclusive as to the application parameters, due to the discrepancy; however all demonstrate the effectiveness of LLLT. According to the data presented, it was concluded that LLLT has positive effects on the control of analgesia for neuropathic pain, but further studies with high scientific rigor are needed in order to define treatment protocols that optimize the action LLLT in neuropathic pain. Article Published Date : Oct 31, 2016

Low-level laser therapy/photobiomodulation in the management of side effects of chemoradiation therapy in head and neck cancer: part 2: proposed applications and treatment protocols. 📎

Abstract Title: Low-level laser therapy/photobiomodulation in the management of side effects of chemoradiation therapy in head and neck cancer: part 2: proposed applications and treatment protocols. Abstract Source: Support Care Cancer. 2016 Mar 17. Epub 2016 Mar 17. PMID: 26984249 Abstract Author(s): Judith A E M Zecha, Judith E Raber-Durlacher, Raj G Nair, Joel B Epstein, Sharon Elad, Michael R Hamblin, Andrei Barasch, Cesar A Migliorati, Dan M J Milstein, Marie-Thérèse Genot, Liset Lansaat, Ron van der Brink, Josep Arnabat-Dominguez, Lisette van der Molen, Irene Jacobi, Judi van Diessen, Jan de Lange, Ludi E Smeele, Mark M Schubert, René-Jean Bensadoun Article Affiliation: Judith A E M Zecha Abstract: PURPOSE: There is a large body of evidence supporting the efficacy of low-level laser therapy (LLLT), more recently termed photobiomodulation (PBM) for the management of oral mucositis (OM) in patients undergoing radiotherapy for head and neck cancer (HNC). Recent advances in PBM technology, together with a better understanding of mechanisms involved and dosimetric parameters may lead to the management of a broader range of complications associated with HNC treatment. This could enhance patient adherence to cancer therapy, and improve quality of life and treatment outcomes. The mechanisms of action, dosimetric, and safety considerations for PBM have been reviewed in part 1. Part 2 discusses the head and neck treatment side effects for which PBM may prove to be effective. In addition, PBM parameters for each of these complications are suggested and future research directions are discussed. METHODS: Narrative review and presentation of PBM parameters are based on current evidence and expert opinion. RESULTS: PBM may have potential applications in the management of a broad range of side effects of (chemo)radiation therapy (CRT) in patients being treated for HNC. For OM management, optimal PBM parameters identified were as follows: wavelength, typically between 633 and 685 nm or 780-830 nm; energy density, laser or light-emitting diode (LED) output between 10 and 150 mW; dose, 2-3 J (J/cm(2)), and no more than 6 J/cm(2) on the tissue surface treated; treatment schedule, two to three times a week up to daily; emission type, pulsed (<100 Hz); and route of delivery, intraorally and/or transcutaneously. To facilitate further studies, we propose potentially effective PBM parameters for prophylactic and therapeutic use in supportive care for dermatitis, dysphagia, dry mouth, dysgeusia, trismus, necrosis, lymphedema, and voice/speech alterations. CONCLUSION: PBM may have a role in supportive care for a broad range of complications associated with the treatment of HNC with CRT. The suggested PBM irradiation and dosimetric parameters, which are potentially effective for these complications, are intended to provide guidance for well-designed future studies. It is imperative that such studies include elucidating the effects of PBM on oncology treatment outcomes. Article Published Date : Mar 16, 2016

Efficacy of low-level laser therapy in temporomandibular disorders: A systematic review. 📎

Abstract Title: Efficacy of low-level laser therapy in temporomandibular disorders: A systematic review. Abstract Source: Natl J Maxillofac Surg. 2016 Jan-Jun;7(1):62-66. PMID: 28163481 Abstract Author(s): Deepankar Shukla, M R Muthusekhar Article Affiliation: Deepankar Shukla Abstract: PURPOSE: The aim of this systematic review was to assess the efficacy of low-level laser therapy (LLLT) in patients with temporomandibular disorders (TMDs). METHODS: Medline search was done from 1997 to 2011 using search terms appropriate to establishing a relation between LLLT and TMD. Only randomized controlled trials were included in this study. Outcome variables related to pain, muscle tenderness, mandibular movements, and Electromyographic (EMG) activity were considered. Of the 242 articles examined, 13 were finally included in the critical analysis conducted as a part of the present systematic review. RESULTS: Of the 242 titles reviewed, only 13 articles were considered eligible. 7 articles showed significant improvement in the study group, whereas 5 showed no significant improvement between the study and control groups. The primary outcome of most of the studies was pain. Other variables considered were muscle tenderness, mandibular movements; EMG activity was considered. CONCLUSION: Our results have shown that LLLT seems to be effective in reducing pain in TMD's. It may be a treatment option for patients with an interest in a noninvasive, complementary therapy. Article Published Date : Dec 31, 2015

The relative antimicrobial effect of blue 405 nm LED and blue 405 nm laser on methicillin-resistant Staphylococcus aureus in vitro.

Abstract Title: The relative antimicrobial effect of blue 405 nm LED and blue 405 nm laser on methicillin-resistant Staphylococcus aureus in vitro. Abstract Source: Lasers Med Sci. 2015 Sep 11. Epub 2015 Sep 11. PMID: 26362142 Abstract Author(s): Daniela Santos Masson-Meyers, Violet Vakunseh Bumah, Gabriel Biener, Valerica Raicu, Chukuka Samuel Enwemeka Article Affiliation: Daniela Santos Masson-Meyers Abstract: It has long been argued that light from a laser diode is superior to light from a light-emitting diode (LED) in terms of its effect on biological tissues. In order to shed light on this ongoing debate, we compared the antimicrobial effect of light emitted from a 405-nm LED with that of a 405-nm laser on methicillin-resistant Staphylococcus aureus (MRSA) at comparable fluences. We cultured 5 × 10(6) CFU/ml MRSA on tryptic soy agar and then irradiated culture plates once, twice, or thrice with either LED or laser light using 40, 54, 81, or 121 J/cm(2) fluence at 15-, 30-, or 240-min time interval between irradiation. Cultures were incubated immediately after irradiation at 37 °C for 24 h before imaging and counting remnant bacterial colonies. Regardless of the device used, LED or laser, irradiation at each fluence resulted in statistically significant bacterial growth suppression compared to non-irradiated controls (p < 0.0001). The antimicrobial effect of both light sources, LED and laser, was not statistically different at each fluence in 35 of the 36 experimental trials. Bacterial growth suppression achieved with either source of light increased with repeated irradiation, particularly at the 15- or 30-min treatment time interval. Thus, we conclude that the antimicrobial effect of 405-nm laser and 405-nm LED on MRSA is similar; neither has a superior antimicrobial effect when compared to the other. Article Published Date : Sep 10, 2015

Systematic review of laser and other light therapy for the management of oral mucositis in cancer patients.

Abstract Title: Systematic review of laser and other light therapy for the management of oral mucositis in cancer patients. Abstract Source: Support Care Cancer. 2013 Jan ;21(1):333-41. Epub 2012 Sep 22. PMID: 23001179 Abstract Author(s): Cesar Migliorati, Ian Hewson, Rajesh V Lalla, Heliton Spindola Antunes, Cherry L Estilo, Brian Hodgson, Nilza Nelly Fontana Lopes, Mark M Schubert, Joanne Bowen, Sharon Elad, Article Affiliation: Cesar Migliorati Abstract: BACKGROUND: The aim of this study was to review the available literature and define clinical practice guidelines for the use of laser and other light therapies for the prevention and treatment of oral mucositis. METHODS: A systematic review was conducted by the Mucositis Study Group of the Multinational Association of Supportive Care in Cancer/International Society of Oral Oncology. The body of evidence for each intervention, in each cancer treatment setting, was assigned an evidence level. Based on the evidence level, one of the following three guideline determinations was possible: recommendation, suggestion, and no guideline possible. RESULTS: A new recommendation was made for low-level laser (wavelength at 650 nm, power of 40 mW, and each square centimeter treated with the required time to a tissue energy dose of 2 J/cm(2) (2 s/point)) for the prevention of oral mucositis in adult patients receiving hematopoietic stem cell transplantation conditioned with high-dose chemotherapy, with or without total body irradiation. A new suggestion was made for low-level laser (wavelength around 632.8 nm) for the prevention of oral mucositis in patients undergoing radiotherapy, without concomitant chemotherapy, for head and neck cancer. No guideline was possible in other populations and for other light sources due to insufficient evidence. CONCLUSIONS: The increasing evidence in favor of low-level laser therapy allowed for the development of two new guidelines supporting this modality in the populations listed above. Evidence for other populations was also generally encouraging over a range of wavelengths and intensities. However, additional well-designed research is needed to evaluate the efficacy of laser and other light therapies in various cancer treatment settings. Article Published Date : Dec 31, 2012

Chapter 25: Phototherapy in peripheral nerve injury: effects on muscle preservation and nerve regeneration.

Abstract Title: Chapter 25: Phototherapy in peripheral nerve injury: effects on muscle preservation and nerve regeneration. Abstract Source: Int Rev Neurobiol. 2009;87:445-64. PMID: 19682654 Abstract Author(s): Shimon Rochkind, Stefano Geuna, Asher Shainberg Article Affiliation: Division of Peripheral Nerve Reconstruction, Department of Neurosurgery, Tel Aviv Sourasky Medical Center, Tel Aviv University, Israel. Abstract: Posttraumatic nerve repair and prevention of muscle atrophy represent a major challenge of restorative medicine. Considerable interest exists in the potential therapeutic value of laser phototherapy for restoring or temporarily preventing denervated muscle atrophy as well as enhancing regeneration of severely injured peripheral nerves. Low-power laser irradiation (laser phototherapy) was applied for treatment of rat denervated muscle in order to estimate biochemical transformation on cellular and tissue levels, as well as on rat sciatic nerve model after crush injury, direct or side-to-end anastomosis, and neurotube reconstruction. Nerve cells' growth and axonal sprouting were investigated in embryonic rat brain cultures. The animal outcome allowed clinical double-blind, placebo-controlled randomized study that measured the effectiveness of 780-nm laser phototherapy on patients suffering from incomplete peripheral nerve injuries for 6 months up to several years. In denervated muscles, animal study suggests that the function of denervated muscles can be partially preserved by temporary prevention of denervation-induced biochemical changes. The function of denervated muscles can be restored, not completely but to a very substantial degree, by laser treatment initiated at the earliest possible stage post injury. In peripheral nerve injury, laser phototherapy has an immediate protective effect. It maintains functional activity of the injured nerve for a long period, decreases scar tissue formation at the injury site, decreases degeneration in corresponding motor neurons of the spinal cord, and significantly increases axonal growth and myelinization. In cell cultures, laser irradiation accelerates migration, nerve cell growth, and fiber sprouting. In a pilot, clinical, double-blind, placebo-controlled randomized study in patients with incomplete long-term peripheral nerve injury, 780-nm laser irradiation can progressively improve peripheral nerve function, which leads to significant functional recovery. A 780-nm laser phototherapy temporarily preserves the function of a denervated muscle, and accelerates and enhances axonal growth and regeneration after peripheral nerve injury or reconstructive procedures. Laser activation of nerve cells, their growth, and axonal sprouting can be considered as potential treatment for neural injury. Animal and clinical studies show the promoting action of phototherapy on peripheral nerve regeneration, which makes it possible to suggest that the time for broader clinical trials has come. Article Published Date : Jan 01, 2009

Stem cell proliferation under low intensity laser irradiation: a preliminary study.

Abstract Title: Stem cell proliferation under low intensity laser irradiation: a preliminary study. Abstract Source: Lasers Surg Med. 2008 Aug;40(6):433-8. PMID: 18649378 Abstract Author(s): Fernanda de P Eduardo, Daniela F Bueno, Patricia M de Freitas, Márcia Martins Marques, Maria Rita Passos-Bueno, Carlos de P Eduardo, Mayana Zatz Article Affiliation: Hospital Israelita Albert Einstein, Unit of Bone Marrow Transplantation, São Paulo 05651-901, SP, Brazil. Abstract: BACKGROUND AND OBJECTIVES: Phototherapy with low intensity laser irradiation has shown to be effective in promoting the proliferation of different cells. The aim of this in vitro study was to evaluate the potential effect of laser phototherapy (660 nm) on human dental pulp stem cell (hDPSC) proliferation. STUDY DESIGN/MATERIALS AND METHODS: The hDPSC cell strain was used. Cells cultured under nutritional deficit (10% FBS) were either irradiated or not (control) using two different power settings (20 mW/6 seconds to 40 mW/3 seconds), with an InGaAIP diode laser. The cell growth was indirectly assessed by measuring the cell mitochondrial activity through the MTT reduction-based cytotoxicity assay. RESULTS: The group irradiated with the 20 mW setting presented significantly higher MTT activity at 72 hours than the other two groups (negative control--10% FBS--and lased 40 mW with 3 seconds exposure time). After 24 hours of the first irradiation, cultures grown under nutritional deficit (10% FBS) and irradiated presented significantly higher viable cells than the non-irradiated cultures grown under the same nutritional conditions. CONCLUSIONS: Under the conditions of this study it was possible to conclude that the cell strain hDPSC responds positively to laser phototherapy by improving the cell growth when cultured under nutritional deficit conditions. Thus, the association of laser phototherapy and hDPSC cells could be of importance for future tissue engineering and regenerative medicine. Moreover, it opens the possibility of using laser phototherapy for improving the cell growth of other types of stem cells. Article Published Date : Aug 01, 2008
Therapeutic Actions Laser Treatment - Low-Level

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Current treatments of acne: Medications, lights, lasers, and a novel 650-μs 1064-nm Nd: YAG laser.

Related Articles Current treatments of acne: Medications, lights, lasers, and a novel 650-μs 1064-nm Nd: YAG laser. J Cosmet Dermatol. 2017 Sep;16(3):303-318 Authors: Gold MH, Goldberg DJ, Nestor MS Abstract The treatment of acne, especially severe acne, remains a challenge to dermatologists. Therapies include retinoids, antibiotics, hormones, lights, lasers, and various combinations of these modalities. Acne is currently considered a chronic rather than an adolescent condition. The appropriate treatment depends on the patient and the severity of disease. The purpose of this study was to review current therapies for acne of all severities and to introduce the 650-μs 1064-nm laser for the treatment of acne. PMID: 28703382 [PubMed - indexed for MEDLINE]

Photoactivation of ERK/CREB/VMAT2 pathway attenuates MPP+-induced neuronal injury in a cellular model of Parkinson's disease.

Related Articles Photoactivation of ERK/CREB/VMAT2 pathway attenuates MPP+-induced neuronal injury in a cellular model of Parkinson's disease. Cell Signal. 2017 Sep;37:103-114 Authors: Gu X, Liu L, Shen Q, Xing D Abstract The vesicular monoamine transporter 2 (VMAT2) pumps dopamine from cytoplasm into synaptic vesicles for subsequent release, and the deficits of VMAT2 has been implicated in the dopaminergic neuronal cell loss which is considered as a typical pathological feature of Parkinson's disease (PD). Low-power laser irradiation (LPLI), a potent noninvasive physiotherapy approach, is capable of penetrating into nerve tissue to exert beneficial effects such as promoting nerve regeneration and ATP production. In the present study, we demonstrated that LPLI protects against MPP+-induced neurotoxicity via upregulation of VMAT2 in SH-SY5Y human dopaminergic neuroblastoma cells. The photoactivation of ERK phosphorylated cAMP-response element binding protein (CREB) at Ser133, and thus increased the ability of CREB binding to the promoter region of VMAT2, leading to elevated VMAT2 expression, which contributes to dopamine release and cell survival. Taken together, for the first time to our knowledge, the results showed that LPLI attenuates MPP+-induced neurotoxicity through activation of ERK/CREB/VMAT2 pathway, suggesting that the manipulation of VMAT2 by LPLI may provide a potential therapeutic strategy for PD. PMID: 28627368 [PubMed - indexed for MEDLINE]