CYBERMED LIFE - ORGANIC  & NATURAL LIVING

Cybermedlife - Therapeutic Actions Sunlight exposure

Sun Exposure across the Life Course Significantly Modulates Early Multiple Sclerosis Clinical Course. 📎

Abstract Title: Sun Exposure across the Life Course Significantly Modulates Early Multiple Sclerosis Clinical Course. Abstract Source: Front Neurol. 2018 ;9:16. Epub 2018 Feb 1. PMID: 29449827 Abstract Author(s): Steve Simpson, Ingrid van der Mei, Robyn M Lucas, Anne-Louise Ponsonby, Simon Broadley, Leigh Blizzard, , Bruce Taylor Article Affiliation: Steve Simpson Abstract: Background: Low vitamin D and/or sun exposure have been associated with increased risk of multiple sclerosis (MS) onset. However, comparatively, few studies have prospectively examined associations between these factors and clinical course. Objectives: To evaluate the association of sun exposure parameters and vitamin D levels with conversion to MS and relapse risk in a prospectively monitored cohort of 145 participants followed after a first demyelinating event up to 5-year review (AusLong Study). Methods: Sun exposure prior to and after onset measured by annual questionnaire; ultraviolet radiation (UVR)"load"estimated by location of residence over the life course and ambient UVR levels. Serum 25-hydroxyvitamin D [25(OH)D] concentrations measured at baseline, 2/3-year, and 5-year review. MS conversion and relapse assessed by neurologist assessment and medical record review. Results: Over two-thirds (69%) of those followed to 5-year review (100/145) converted to MS, with a total of 252 relapses. Higher pre-MS onset sun exposure was associated with reduced risk of MS conversion, with internal consistency between measures and dose-response relationships. Analogous associations were also seen with risk of relapse, albeit less strong. No consistent associations were observed between postonset sun exposure and clinical course, however. Notably, those who increased their sun exposure during follow-up had significantly reduced hazards of MS conversion and relapse. Serum 25(OH)D levels and vitamin D supplementation were not associated with conversion to MS or relapse hazard. Conclusion: We found that preonset sun exposure was protective against subsequent conversion to MS and relapses. While consistent associations between postonset sun exposure or serum 25(OH)D level and clinical course were not evident, possibly masked by behavior change, those participants who markedly increased their sun exposure demonstrated a reduced MS conversion and relapse hazard, suggesting beneficial effects of sun exposure on clinical course. Article Published Date : Dec 31, 2017

Preharvest Ultraviolet C Irradiation Increased the Level of Polyphenol Accumulation and Flavonoid Pathway Gene Expression in Strawberry Fruit.

Abstract Title: Preharvest Ultraviolet C Irradiation Increased the Level of Polyphenol Accumulation and Flavonoid Pathway Gene Expression in Strawberry Fruit. Abstract Source: J Agric Food Chem. 2017 Nov 22 ;65(46):9970-9979. Epub 2017 Nov 8. PMID: 29091440 Abstract Author(s): Yanqun Xu, Marie Thérèse Charles, Zisheng Luo, Benjamin Mimee, Pierre-Yves Veronneau, Daniel Rolland, Dominique Roussel Article Affiliation: Yanqun Xu Abstract: Preharvest ultraviolet C (UV-C) irradiation is an innovative approach for increasing the bioactive phytochemical content of strawberries to increase the disease resistance and nutritional value. This study investigated the changes in individual flavonoids in strawberry developed with three different cumulative doses of preharvest UV-C treatment (low, 9.6 kJ m; middle, 15 kJ m; and high , 29.4 kJ m). Significant accumulation (p<0.05) of phenolics (25-75% increase), namely, cyanidin 3-glucoside, pelargonidin 3-glucoside/rutinoside, glucoside and glucuronide of quercetin and kaempferol, and ellagic acid, was found in the fruit subjected to low and middle supplemental doses of UV-C radiation. The expression of the flavonoid pathway structural genes, i.e., FaCHS1, FaCHI, FaFHT, FaDFR, FaFLS, and FaFGT, was upregulated in the low- and middle-dose groups, while the early stage genes were not affected by the high dose. FaMYB1 was also relatively enhanced in the low- and middle-dose groups, while FaASR was upregulated in only the low-dose group. Hormetic preharvest UV-C dose ranges for enhancing the polyphenol content of strawberries were established for the first time. Article Published Date : Nov 21, 2017

Comparison of sun exposure versus vitamin D supplementation for pregnant women with vitamin D deficiency.

Abstract Title: Comparison of sun exposure versus vitamin D supplementation for pregnant women with vitamin D deficiency. Abstract Source: J Matern Fetal Neonatal Med. 2017 Nov 15:1-111. Epub 2017 Nov 15. PMID: 29141476 Abstract Author(s): Maryam Hajhashemi, Azadeh Khorsandi, Fedyeh Haghollahi Article Affiliation: Maryam Hajhashemi Abstract: INTRODUCTION: Maternal vitamin D deficiency is widespread health problem which is more important in pregnant women which affects fetus growth and bone development. The aim of this study was to evaluate the effect of sun exposure versus vitamin D supplementation for pregnant women with vitamin D deficiency. MATERIALS AND METHODS: This prospective clinical trial was performed on 87 pregnant women with vitamin D deficiency. Group A was treated with vitamin D 4000 IU per day for 10 weeks, while group B was recommended for sun exposure for 30 minutes daily (30% body surface area) for 10 weeks in summer and between 10 am-4 pm in direct sunlight. After the delivery, 25-hydroxyvitamin D3 levels were measured in the same previous center. Moreover, weight, height and head circumference of fetus were measured at delivery in both groups and compared with each other. RESULTS: After 10-week intervention, 25-hydroxyvitamin D3 levels was significantly higher in group treated with vitamin D as compared to sun expose group (31.27 versus 19.79 ng/ml). (p<0.001). However, height (p = 0.118), weight (p = 0.245) and head circumference (p = 0.681) of infants in both groups did not show significant differences. CONCLUSION: Vitamin D supplementation is more effective than sun exposure in increasing 25-hydroxyvitamin D3 in pregnant women with vitamin D deficiency. Article Published Date : Nov 14, 2017

Prevention of transfusion-associated graft-versus-host disease with pathogen-reduced platelets with amotosalen and ultraviolet A light: a review.

Abstract Title: Prevention of transfusion-associated graft-versus-host disease with pathogen-reduced platelets with amotosalen and ultraviolet A light: a review. Abstract Source: Vox Sang. 2017 Oct ;112(7):607-613. Epub 2017 Aug 18. PMID: 28833219 Abstract Author(s): J Cid Article Affiliation: J Cid Abstract: BACKGROUND AND OBJECTIVES: Transfusion-associated graft-versus-host disease (TA-GVHD) is a serious complication of blood component transfusion therapy, caused by donor T lymphocytes.γ-Irradiation or pathogen inactivation methods, capable of inactivating proliferating T cells in blood components, should be selected to prevent TA-GVHD. This review summarizes the published evidence to support the use of pathogen-reduced platelets with amotosalen (150 μm) and ultraviolet A light(UVA, 320-400 nm, 3 J/cm) for preventing TA-GVHD. MATERIALS AND METHODS: Available literature on the use of pathogen-reduced platelets to prevent TA-GVHD was reviewed. RESULTS: Observational studies, animal models, in vitro studies and mechanistic studies of pathogen-reduced platelets with amotosalen and UVA light showed that inactivation of T cells are equal or even superior toγ-irradiation. CONCLUSION: Pathogen-reduced platelets with amotosalen and UVA light can be used as a measure to prevent TA-GVHD. Article Published Date : Sep 30, 2017

Ultraviolet-A1 irradiation therapy for systemic lupus erythematosus. 📎

Abstract Title: Ultraviolet-A1 irradiation therapy for systemic lupus erythematosus. Abstract Source: Lupus. 2017 Oct ;26(12):1239-1251. Epub 2017 May 8. PMID: 28480786 Abstract Author(s): H McGrath Article Affiliation: H McGrath Abstract: Systemic lupus erythematosus (lupus, SLE) is a chronic autoimmune disease characterized by the production of autoantibodies, which bind to antigens and are deposited within tissues to fix complement, resulting in widespread systemic inflammation. The studies presented herein are consistent with hyperpolarized, adenosine triphosphate (ATP)-deficient mitochondria being central to the disease process. These hyperpolarized mitochondria resist the depolarization required for activation-induced apoptosis. The mitochondrial ATP deficits add to this resistance to apoptosis and also reduce the macrophage energy that is needed to clear apoptotic bodies. In both cases, necrosis, the alternative pathway of cell death, results. Intracellular constituents spill into the blood and tissues, eliciting inflammatory responses directed at their removal. What results is"autoimmunity."Ultraviolet (UV)-A1 photons have the capacity to remediate this aberrancy. Exogenous exposure to low-dose, full-body, UV-A1 radiation generates singlet oxygen. Singlet oxygen has two major palliative actions in patients with lupus and the UV-A1 photons themselves have several more. Singlet oxygen depolarizes the hyperpolarized mitochondrion, triggering non-ATP-dependent apoptosis that deters necrosis. Next, singlet oxygen activates the gene encoding heme oxygenase (HO-1), a major governor of systemic homeostasis. HO-1 catalyzes the degradation of the oxidant heme into biliverdin (converted to bilirubin), Fe, and carbon monoxide (CO), the first three of these exerting powerful antioxidant effects, and in conjunction with a fourth, CO, protecting against injury to the coronary arteries, the central nervous system, and the lungs. The UV-A1 photons themselves directly attenuate disease in lupus by reducing B cell activity, preventing the suppression of cell-mediated immunity, slowing an epigenetic progression toward SLE, and ameliorating discoid and subacute cutaneous lupus. Finally, a combination of these mechanisms reduces levels of anticardiolipin antibodies and protects during lupus pregnancy. Capping all of this is that UV-A1 irradiation is an essentially innocuous, highly manageable, and comfortable therapeutic agency. Article Published Date : Sep 30, 2017

Demethoxycurcumin in combination with ultraviolet radiation B induces apoptosis through the mitochondrial pathway and caspase activation in A431 and HaCaT cells. 📎

Abstract Title: Demethoxycurcumin in combination with ultraviolet radiation B induces apoptosis through the mitochondrial pathway and caspase activation in A431 and HaCaT cells. Abstract Source: Tumour Biol. 2017 Jun ;39(6):1010428317706216. PMID: 28618944 Abstract Author(s): Yong Xin, Qian Huang, Pei Zhang, Wen Wen Guo, Long Zhen Zhang, Guan Jiang Article Affiliation: Yong Xin Abstract: Photodynamic therapy is widely used in the clinical treatment of tumors, especially skin cancers. It has been reported that the photosensitizer curcumin, in combination with ultraviolet radiation B, induces HaCaT cell apoptosis, and this effect may be due to the activation of caspase pathways. In this study, we examined the photodynamic effects of demethoxycurcumin, a more stable analogue of curcumin, to determine whether it could induce apoptosis in skin cancer cells. We investigated the effects of a combination of ultraviolet radiation B and demethoxycurcumin on apoptotic cell death in A431 and HaCaT cells and determined the molecular mechanism of action. Our results showed increased apoptosis with a combination of ultraviolet radiation B with demethoxycurcumin, as compared to ultraviolet radiation B or demethoxycurcumin alone. The combination of ultraviolet radiation B irradiation with demethoxycurcumin synergistically induced apoptotic cell death in A431 and HaCaT cells through activation of p53 and caspase pathways, as well as through upregulation of Bax and p-p65 expression and downregulation of Bcl-2, Mcl-1, and nuclear factor-κB expression. In addition, we found that reactive oxygen species significantly increased with treatment, and mitochondrial membrane potential depolarization was remarkably enhanced. In conclusion, our data indicate that demethoxycurcumin may be a promising photosensitizer for use in photodynamic therapy to induce apoptosis in skin cancer cells. Article Published Date : May 31, 2017

Short-term UVB irradiation significantly increases vitamin D serum concentration in obese patients: a clinical pilot study.

Abstract Title: Short-term UVB irradiation significantly increases vitamin D serum concentration in obese patients: a clinical pilot study. Abstract Source: Endocrine. 2017 Apr ;56(1):186-195. Epub 2017 Feb 10. PMID: 28188479 Abstract Author(s): Alexander Obbarius, Heike Berger, Andreas Stengel, Carmen Garcia, Felix Fischer, Tobias Hofmann, Matthias Rose, Ralf Uebelhack Article Affiliation: Alexander Obbarius Abstract: PURPOSE: Deficiency of vitamin D is very common in obese people and treatment by oral supplementation is not effective in all patients. This exploratory pilot study investigated the influence of different doses of short-term ultraviolet B irradiation on serum 25-hydroxyvitamin-D(25D) and 1,25-dihydroxyvitamin-D(1,25D) levels in obese compared to normal weight subjects and obese controls. METHODS: Participants with skin types II and III (Fitzpatrick skin classification) were assigned to six groups including four intervention groups receiving irradiation (three groups of obese and one group of normal weight subjects) and two control groups without treatment (obese and normal weight). Intervention groups received three sessions of whole body UVB irradiation of three different doses (cumulative doses over three sessions: 0.28, 0.70, 1.75 minimal erythema dose) within 1 week of intervention. Serum 25D and 1,25D were measured at baseline and after irradiation. Outcome differences between groups were analyzed using a linear model. RESULTS: Serum 25D levels increased significantly in obese (+23.6 and +26.7%, respectively, p = 0.01) and normal weight (+15.6%, p = 0.02) intervention groups who received medium and high doses of ultraviolet B irradiation compared to control groups (+3.5 and -4.0%, respectively, p = 1.0). The increase in obese patients was 51.4% greater compared to normal weight controls irradiated with equal ultraviolet B doses. Low-level ultraviolet irradiation did not result in a significant change in serum 25D (+7.0%, p = 0.61). We did not detect any significant differences of 1,25D between groups (p = 0.25). CONCLUSIONS: The current study indicates that short-term ultraviolet B irradiation increases 25D levels in obese patients. Article Published Date : Mar 31, 2017

Ultraviolet B decreases DNA methylation level of CD4+ T cells in patients with systemic lupus erythematosus.

Abstract Title: Ultraviolet B decreases DNA methylation level of CD4+ T cells in patients with systemic lupus erythematosus. Abstract Source: Inflammopharmacology. 2017 Apr ;25(2):203-210. Epub 2017 Feb 11. PMID: 28190128 Abstract Author(s): Min Zhang, Xuan Fang, Guo-Sheng Wang, Yan Ma, Li Jin, Xiao-Mei Li, Xiang-Pei Li Article Affiliation: Min Zhang Abstract: OBJECTIVE: In the present study, DNA methylation level of CD4+ T cells exposed to ultraviolet B (UVB) was investigated and its potential mechanisms were also explored. METHODS: CD4+ T cells from 12 cases of healthy subjects and 33 cases of SLE patients were isolated and exposed to different dosages (0, 50, 100 mJ/cm) of UVB. Further, SLE patients were divided into two groups: active SLE group (22 cases, SLEDAI scores >4) and inactive SLE group (11 cases, SLEDAI scores ≤4). DNA methylation was evaluated by the Methylamp™ Global DNA Methylation Quantification Ultra Kit. The mRNA and protein expression levels of DNA methyltransferases (DNMT1 and DNMT3A) were detected by real-time PCR and western blot, respectively. RESULTS: The levels of DNA methylation and DNMT3A mRNA in SLE patients were significantly decreased compared with those in healthy subjects at baseline. After different dosages of ultraviolet irradiation (0, 50 and 100 mJ/cm), DNA methylation levels of CD4+ T cells were all reduced in a dose-dependent manner in three subgroups. Additionally, 100 mJ/cmultraviolet irradiation in active SLE group contributed to a significant decrease of both DNA methylation and DNMT3A mRNA levels in CD4+ T cells. UVB exposure had no significant effects on expression levels of DNMT1 mRNA and protein and DNMT3A protein. CONCLUSION: UVB decreases DNA methylation level of CD4+ T cells in SLE patients probably via inhibiting DNMT3A mRNA expression level, which needs to be further explored. Article Published Date : Mar 31, 2017

Ultraviolet radiation, vitamin D and the development of obesity, metabolic syndrome and type-2 diabetes.

Abstract Title: Ultraviolet radiation, vitamin D and the development of obesity, metabolic syndrome and type-2 diabetes. Abstract Source: Photochem Photobiol Sci. 2017 Mar 16 ;16(3):362-373. PMID: 28009891 Abstract Author(s): Shelley Gorman, Robyn M Lucas, Aidan Allen-Hall, Naomi Fleury, Martin Feelisch Article Affiliation: Shelley Gorman Abstract: Obesity is increasing in prevalence in many countries around the world. Its causes have been traditionally ascribed to a model where energy intake exceeds energy consumption. Reduced energy output in the form of exercise is associated with less sun exposure as many of these activities occur outdoors. This review explores the potential for ultraviolet radiation (UVR), derived from sun exposure, to affect the development of obesity and two of its metabolic co-morbidities, type-2 diabetes and metabolic syndrome. We here discuss the potential benefits (or otherwise) of exposure to UVR based on evidence from pre-clinical, human epidemiological and clinical studies and explore and compare the potential role of UVR-induced mediators, including vitamin D and nitric oxide. Overall, emerging findings suggest a protective role for UVR and sun exposure in reducing the development of obesity and cardiometabolic dysfunction, but more epidemiological and clinical research is required that focuses on measuring the direct associations and effects of exposure to UVR in humans. Article Published Date : Mar 15, 2017

Does the Access to Sun Exposure Ensure Adequate Levels of 25-Hydroxyvitamin D? 📎

Abstract Title: Does the Access to Sun Exposure Ensure Adequate Levels of 25-Hydroxyvitamin D? Abstract Source: Rev Bras Ginecol Obstet. 2017 Mar 15. Epub 2017 Mar 15. PMID: 28297731 Abstract Author(s): Ênio Luis Damaso, Francisco José Albuquerque de Paula, Silvio Antônio Franceschini, Carolina Sales Vieira, Rui Alberto Ferriani, Marcos Felipe Silva de Sá, Lucia Alves da Silva Lara Article Affiliation: Ênio Luis Damaso Abstract: Objectives To assess the prevalence of hypovitaminosis D, altered arterial blood pressure, and serum levels of glucose and lipids in community-dwelling women in the city of Ribeirão Preto, in the southeast of Brazil. Methods This was a cross-sectional study of women aged 40-70 years old. Calcium intake and level of sun exposure were assessed by means of a questionnaire. A blood sample was used to determine glucose, lipid profile and 25-hydroxyvitamin D (25[OH]D) concentration. Results Ninety-one women were enrolled (age = 54.2 ± 7.1 years). The mean serum 25(OH)D concentration was 25.7 ± 8.9 ng/mL. A total of 24 (26.4%) women had 25(OH)D levels < 20 ng/mL. Seventy women (76.9%) had 25(OH)D levels < 30 ng/mL. Seventy-five women (90.4%) had inadequate calcium intake, and 61 women (67%) had appropriate sun exposure, 49 of whom (80.3%) had serum 25(OH)D levels < 30 ng/mL. Conclusion This study indicates that even in community-dwelling women, living in a city with high sun exposure, serum levels of 25(OH)D > 30 ng/ml are hardly reached. Thus, it is probable that other intrinsic factors besides sun exposure may regulate the levels of vitamin D. Article Published Date : Mar 14, 2017

Ultraviolet A eye irradiation ameliorates colon carcinoma induced by azoxymethane and dextran sodium sulfate throughβ-endorphin and methionine-enkephalin.

Abstract Title: Ultraviolet A eye irradiation ameliorates colon carcinoma induced by azoxymethane and dextran sodium sulfate throughβ-endorphin and methionine-enkephalin. Abstract Source: Photodermatol Photoimmunol Photomed. 2017 Mar ;33(2):84-91. Epub 2017 Jan 19. PMID: 28039905 Abstract Author(s): Keiichi Hiramoto, Satoshi Yokoyama, Yurika Yamate Article Affiliation: Keiichi Hiramoto Abstract: BACKGROUND: We previously reported that ultraviolet (UV) A eye irradiation reduces the ulcerative colitis induced by dextran sodium sulfate (DSS). This study examined the effects of UVA on colon carcinoma induced by azoxymethane (AOM) and DSS. METHODS: We irradiated the eyes of ICR mice with UVA at a dose of 110 kJ/musing an FL20SBLB-A lamp for the experimental period. RESULTS: In mice treated with these drugs, the symptom of colon carcinoma was reduced by UVA eye irradiation. The levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α in the blood were increased in AOM + DSS-treated mice; however, those levels were reduced by UVA eye irradiation. The expression of β-endorphin, methionine-enkephalin (OGF), μ-opioid receptor, and opioid growth factor receptor (OGFR) of the colon was increased in the AOM + DSS-treated mice, andthese levels were increased further following UVA eye irradiation. When β-endorphin inhibitor was administered, the ameliorative effect of UVA eye irradiation was reduced, and the effect of eye irradiation disappeared entirely following the administration of naltrexone (inhibitor of both opioid receptor and OGFR). CONCLUSIONS: These results suggested that UVA eye irradiation exerts major effects on AOM + DSS-induced colon carcinoma. Article Published Date : Feb 28, 2017

The Preventive Effect of Coffee Compounds on Dermatitis and Epidermal Pigmentation after Ultraviolet Irradiation in Mice. 📎

Abstract Title: The Preventive Effect of Coffee Compounds on Dermatitis and Epidermal Pigmentation after Ultraviolet Irradiation in Mice. Abstract Source: Skin Pharmacol Physiol. 2017 ;30(1):24-35. Epub 2017 Feb 3. PMID: 28152530 Abstract Author(s): Yurika Yamate, Keiichi Hiramoto, Eisuke F Sato Article Affiliation: Yurika Yamate Abstract: BACKGROUND: Ultraviolet (UV) irradiation is well known to promote inflammation and pigmentation of skin. UVB mainly affects dermatitis and pigmentation. Coffee contains a number of polyphenols, such as caffeic acid (CA) and chlorogenic acid (CGA) but their in vivo bioactivity for photobiology remains unclear. METHODS: C57BL/6j male mice were irradiated with UVB (1.0 kJ/m2/day) for 3 days. Five days after the final session of UVB irradiation, the dorsal skin, ear epidermis, and blood samples were analyzed to investigate the inflammatory factors, melanogenesis factors and related hormones. RESULTS: After the oral administration of CA (100 mg/day) or CGA (100 mg/day) for 8 days, only CA was found to inhibit dermatitis and pigmentation. The pathway by which CA inhibits dermatitis is related to the mitogen-activated protein kinase (MAPK)/extracellular signal regulated kinase (ERK)1/2/cAMP response element binding protein (CREB) pathway. Otherwise, the pathway by which CA inhibits pigmentation is related to the activation of theβ-endorphin-μ-opioid receptor and suppresses the cAMP-microphthalmia-associated transcription factor (MITF) pathway. CONCLUSION: It is suggested that the oral administration of CA prevented dermatitis and pigmentation after UVB irradiation in mice. Article Published Date : Dec 31, 2016

UVB Exposure Prevents Atherosclerosis by Regulating Immunoinflammatory Responses. 📎

Abstract Title: UVB Exposure Prevents Atherosclerosis by Regulating Immunoinflammatory Responses. Abstract Source: Arterioscler Thromb Vasc Biol. 2017 01 ;37(1):66-74. Epub 2016 Oct 20. PMID: 27765767 Abstract Author(s): Naoto Sasaki, Tomoya Yamashita, Kazuyuki Kasahara, Atsushi Fukunaga, Tomoyuki Yamaguchi, Takuo Emoto, Keiko Yodoi, Takuya Matsumoto, Kenji Nakajima, Tomoyuki Kita, Masafumi Takeda, Taiji Mizoguchi, Tomohiro Hayashi, Yoshihiro Sasaki, Mayumi Hatakeyama, Kumiko Taguchi, Ken Washio, Shimon Sakaguchi, Bernard Malissen, Chikako Nishigori, Ken-Ichi Hirata Article Affiliation: Naoto Sasaki Abstract: OBJECTIVE: UVB irradiation is an established treatment for immunoinflammatory cutaneous disorders and has been shown to suppress cutaneous and systemic inflammatory diseases through modulation of the adaptive immune response. However, it remains unknown whether UVB irradiation prevents an immunoinflammatory disease of arteries such as atherosclerosis. APPROACH AND RESULTS: Here, we show that UVB exposure inhibits the development and progression of atherosclerosis in atherosclerosis-prone mice by expanding and enhancing the functional capacity of CD4forkhead box P3regulatory T cells and regulating proatherogenic T-cell responses. Experimental studies in Langerhans cell-depleted mice revealed that epidermal Langerhans cells play a critical role in UVB-dependent induction of CD4forkhead box P3regulatory T cells, suppression of proatherogenic T-cell responses, and prevention of atherosclerotic plaque development. CONCLUSIONS: Our findings suggest the skin immune system as a novel therapeutic target for atherosclerosis and provide a novel strategy for the treatment and prevention of atherosclerosis. Article Published Date : Dec 31, 2016

Exosomes are released by bystander cells exposed to radiation-induced biophoton signals: Reconciling the mechanisms mediating the bystander effect. 📎

Abstract Title: Exosomes are released by bystander cells exposed to radiation-induced biophoton signals: Reconciling the mechanisms mediating the bystander effect. Abstract Source: PLoS One. 2017 ;12(3):e0173685. Epub 2017 Mar 9. PMID: 28278290 Abstract Author(s): Michelle Le, Cristian Fernandez-Palomo, Fiona E McNeill, Colin B Seymour, Andrew J Rainbow, Carmel E Mothersill Article Affiliation: Michelle Le Abstract: OBJECTIVE: The objective of our study was to explore a possible molecular mechanism by which ultraviolet (UV) biophotons could elicit bystander responses in reporter cells and resolve the problem of seemingly mutually exclusive mechanisms of a physical UV signal&a soluble factor-mediated bystander signal. METHODS: The human colon carcinoma cell line, HCT116 p53 +/+, was directly irradiated with 0.5 Gy tritium beta particles to induce ultraviolet biophoton emission. Bystander cells were not directly irradiated but were exposed to the emitted UV biophotons. Medium was subsequently harvested from UV-exposed bystander cells. The exosomes extracted from this medium were incubated with reporter cell populations. These reporter cells were then assayed for clonogenic survival and mitochondrial membrane potential with and without prior treatment of the exosomes with RNase. RESULTS: Clonogenic cell survival was significantly reduced in reporter cells incubated with exosomes extracted from cells exposed to secondarily-emitted UV. These exosomes also induced significant mitochondrial membrane depolarization in receiving reporter cells. Conversely, exosomes extracted from non-UV-exposed cells did not produce bystander effects in reporter cells. The treatment of exosomes with RNase prior to their incubation with reporter cells effectively abolished bystander effects in reporter cells and this suggests a role for RNA in mediating the bystander response elicited by UV biophotons and their produced exosomes. CONCLUSION: This study supports a role for exosomes released from UV biophoton-exposed bystander cells in eliciting bystander responses and also indicates a reconciliation between the UV-mediated bystander effect and the bystander effect which has been suggested in the literature to be mediated by soluble factors. Article Published Date : Dec 31, 2016

Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment: a comparative study between ultraviolet C and ultraviolet B.

Abstract Title: Inactivation of Pseudomonas aeruginosa biofilm after ultraviolet light-emitting diode treatment: a comparative study between ultraviolet C and ultraviolet B. Abstract Source: J Biomed Opt. 2017 06 1 ;22(6):65004. PMID: 28655056 Abstract Author(s): Aikaterini Argyraki, Merete Markvart, Lars Bjørndal, Thomas Bjarnsholt, Paul Michael Petersen Article Affiliation: Aikaterini Argyraki Abstract: The objective of this study was to test the inactivation efficiency of two different light-based treatments, namely ultraviolet B (UVB) and ultraviolet C (UVC) irradiation, on Pseudomonas aeruginosa biofilms at different growth stages (24, 48, and 72 h grown). In our experiments, a type of AlGaN light-emitting diodes (LEDs) was used to deliver UV irradiation on the biofilms. The effectiveness of the UVB at 296 nm and UVC at 266 nm irradiations was quantified by counting colony-forming units. The survival of less mature biofilms (24 h grown) was studied as a function of UV-radiant exposure. All treatments were performed on three different biological replicates to test reproducibility. It was shown that UVB irradiation was significantly more effective than UVC irradiation in inactivating P. aeruginosa biofilms. UVC irradiation induced insignificant inactivation on mature biofilms. The fact that the UVB at 296 nm exists in daylight and has such disinfection ability on biofilms provides perspectives for the treatment of infectious diseases. Article Published Date : Dec 31, 2016
Prev12345Next
Therapeutic Actions Sunlight exposure

NCBI pubmed

Solar UV light regulates flavonoid metabolism in apple (Malus x domestica).

Related Articles Solar UV light regulates flavonoid metabolism in apple (Malus x domestica). Plant Cell Environ. 2018 03;41(3):675-688 Authors: Henry-Kirk RA, Plunkett B, Hall M, McGhie T, Allan AC, Wargent JJ, Espley RV Abstract Ultraviolet-B light (UV-B) is one environmental signal perceived by plants that affects the flavonoid pathway and influences the levels of anthocyanins, flavonols, and proanthocyanidins. To understand the mechanisms underlying UV exposure, apple trees were grown under spectral filters that altered transmission of solar UV light. Fruit analysis showed that UV induced changes in physiology, metabolism, and gene expression levels during development over a season. These changes were sustained after storage. Under low UV, ripening was delayed, fruit size decreased, and anthocyanin and flavonols were reduced. Expression analysis showed changes in response to UV light levels for genes in the regulation and biosynthesis of anthocyanin and flavonols. Transcription of flavonol synthase (FLS), ELONGATED HYPOCOTYL 5 (HY5), MYB10, and MYB22 were down-regulated throughout fruit development under reduced UV. Functional testing showed that the FLS promoter was activated by HY5, and this response was enhanced by the presence of MYB22. The MYB22 promoter can also be activated by the anthocyanin regulator, MYB10. As ambient levels of UV light vary around the globe, this study has implications for future crop production, the quality of which can be determined by the response to UV. PMID: 29315644 [PubMed - indexed for MEDLINE]
Cookies make it easier for us to provide you with our services. With the usage of our services you permit us to use cookies.
More information Ok Decline