The present study evaluates the protective effects of an antioxidant-rich extract of(NAOE) in abnormalities associated with the metabolic syndrome (MetS) in rats. HPTLC of NAOE revealed the presence of 13 total antioxidants, 14 flavonoids, and 10 phenolic acids. Rats administered with fructose (20% /) in drinking water for 45 days to induce abnormalities of MetS received NAOE (200 and 400 mg/kg, po), the standard drug gemfibrozil (60 mg/kg, po), aerobic exercise (AE), and a combination of NAOE 400 mg/kg and AE (NAOEAE) daily for 45 days. All treatments significantly altered the lipid profile and attenuated the fructose-elevated levels of uric acid, C-reactive protein, homocysteine, and marker enzymes (AST, LDH, and CK-MB) in serum and malondialdehyde in the heart and restored the fructose-depleted levels of glutathione and antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase). A significant decrease in blood glucose and insulin levels decreased insulin resistance, and improved glucose tolerance was observed in the treatment animals when compared with the fructose-fed animals. The best mitigation of MetS was shown by the NAOEAE treatment indicating that regular exercise along with adequate consumption of antioxidant-rich foods such as spinach in diet can help control MetS.

CONTEXT:Spinaciaoleracea (spinach) is abundant in antioxidant phytoconstituents, termed as the natural antioxidant mixture (NAO).

OBJECTIVE:This study evaluates the anti-hyperlipidemicand anti-obesity effects of an antioxidant-rich extract of Spinaciaoleracea (NAOE) and aerobic exercise (AE) in rats fed with high fat diet (HFD).

METHODS:Rats received NAOE (200 and 400 mg/kg), the standard drug orlistat (10 mg/kg), AE and NAOEAE (NAOE 400 mg/kg + AE) daily with HFD for 21 d.

RESULTS:Orlistat, NAOE and NAOEAE treatments to HFD-fed rats significantly reduced the HFD-elevated food intake, weight gain, pancreatic lipase activity and lipid peroxidation, and successfully restored the HFD-skewed lipid profile and antioxidant levels.

CONCLUSIONS:It may be concluded that NAOE exhibited a promising anti-hyperlipidemic effect by its inhibitory action on pancreatic lipase. The combination treatment NAOEAE produced the best results indicating the essential role of exercise along with consumption of antioxidant-rich foods in maintaining a normal lipid profile and controlling obesity.

This article provides an overview of the current literature relating to the efficacy of dietary nitrate (NO) ingestion in altering aspects of cardiovascular and metabolic health and exercise capacity in healthy and diseased individuals. The consumption of NO-rich vegetables, such as spinach and beetroot, have been variously shown to promote nitric oxide bioavailability, reduce systemic blood pressure, enhance tissue blood flow, modulate muscle Outilisation and improve exercise tolerance both in normoxia and in hypoxia, as is commonly observed in a number of disease states. NOingestion may, therefore, act as a natural means for augmenting performance and attenuating complications associated with limited Oavailability or transport, hypertension and the metabolic syndrome. Recent studies indicate that dietary NOmight also augment intrinsic skeletal muscle contractility and improve the speed and power of muscle contraction. Moreover, several investigations suggest that NOsupplementation may improve aspects of cognitive performance both at rest and during exercise. Collectively, these observations position NOas more than a putative ergogenic aid and suggest that increasing natural dietary NOintake may act as a prophylactic in countering the predations of senescence and certain cardiovascular-metabolic diseases.

Gonzalez, AM, Accetta, MR, Spitz, RW, Mangine, GT, Ghigiarelli, JJ, and Sell, KM. Red spinach extract supplementation improves cycle time trial performance in recreationally active men and women. J Strength Cond Res XX(X): 000-000, 2019-To examine the effects of short-term red spinach extract (RSE) supplementation on cycling time trial performance, 17 recreationally active men (n = 9, 22.2± 3.8 years) and women (n = 8, 22.8 ± 3.5 years) underwent 2 testing sessions administered in a randomized, counterbalanced, double-blind fashion. Subjects were assigned to supplement daily with 1 g of RSE or placebo (PL) for 7 days priorly and 1 hour before completing a 4-km cycling time trial test. Performance variables (time-to-completion, average power, relative power, cadence, and average speed), subjective measures (perceived exertion and muscle fatigue), heart rate, and blood pressure were assessed during each testing session. Compared to PL, RSE supplementation significantly lowers (p = 0.017, ηp = 0.24) post-exercise diastolic blood pressure (66.1 ± 6.1 vs. 70.1 ± 5.0 mm Hg). Red spinach extract supplementation also significantly improved (p ≤ 0.022, ηp = 0.30-0.37) 4-km completion time (404.6 ± 24.6 vs. 410.6 ± 31.3 seconds), average power (185.9 ± 32.2 vs. 181.6 ±35.1 W), relative power (2.53 ± 0.44 vs. 2.46 ± 0.40 W·kg), and average speed (35.7 ± 2.2 vs. 35.3 ± 2.5 km·h). In addition, significant trial × sex interactions (p ≤ 0.022, ηp = 0.30-0.36) were observed for these performance measures, whereby only women showed significant improvement during RSE compared with PL trials. In conclusion, RSE supplementation significantly reduced time-to-completion, increased measures of power and speed, and lowered post-exercise diastolic blood pressure during a 4-km cycling time trial without altering subjects' perceived exertion or subjective measuresof muscle fatigue. Finally, it is possible that women may be more responsive in regard to increasing performance after supplementation.