Tissue response to root canal irrigation systems in dogs' teeth with apical periodontitis.
Clin Oral Investig. 2015 Jun;19(5):1147-56
Authors: Cohenca N, Romualdo PC, da Silva LA, da Silva RA, de Queiroz AM, De Rossi A, Nelson-Filho P
OBJECTIVES: The aim of this study was to compare the efficacy of apical negative pressure irrigation (ANP) and passive ultrasonic irrigation (PUI) with apical positive pressure irrigation (PP-conventional irrigation) in the periapical repair of dogs' teeth with apical periodontitis.
METHODS: Forty-nine mesial and distal root canals of premolars of three dogs with experimentally induced periapical lesions were subjected to a single-session root canal treatment. The teeth were randomly assigned to three groups: ANP, PUI, and PP (control). After 180 days, the animals were euthanized, and the anatomic pieces were removed and subjected to histotechnical processing for morphological and morphometric histological analyses of hematoxylin and eosin (HE)-stained sections under conventional and fluorescence microscopy. Periapical lesion size before and 180 days after root canal treatment was measured by periapical radiographic examination. Tartrate-resistant acid phosphatase (TRAP) histoenzymology was performed for osteoclast counting and Brown and Brenn staining to assess bacteria. Data were analyzed statistically by the Kruskal-Wallis test (α = 5 %).
RESULT: There were no statistically significant differences among the groups regarding periapical lesion size in the radiographic evaluation (p = 0.91). In the comparison of histopathological parameters, group ANP presented more homogeneous results. There was a statistically significant difference (p = 0.02) between groups ANP and PP, with better results for group ANP in which milder infiltrate inflammatory was observed. No statistically significant difference (p > 0.05) was found among the groups with respect to periodontal ligament space, presence of mineralized tissue resorption, size of the periapical lesions, and number of osteoclasts.
CONCLUSION: Although the three irrigation systems elicited similar periapical tissue response with respect to almost all evaluated parameters, ANP presented the mildest inflammatory infiltrate, suggesting an advantage over PP in the indication for clinical use.
CLINICAL RELEVANCE: Irrigation of the root canal systems is an extremely important step in root canal treatment. Therefore, it is of relevance to evaluate the efficacy of these systems by in vivo experimental models to provide scientific background for the clinical practice.
PMID: 25338736 [PubMed - indexed for MEDLINE]
The effect of photon-initiated photoacoustic streaming, ultrasonically and sonically irrigation techniques on the push-out bond strength of a resin sealer to the root dentin.
Clin Oral Investig. 2015 Jun;19(5):1055-61
Authors: Akcay M, Arslan H, Mese M, Sahin NN
OBJECTIVES: The present study investigated the effects of various irrigation activation techniques, including laser-activated irrigation using a laser with a novel tip design (photon-induced photoacoustic streaming, PIPS) on the bond strength of an epoxy resin-based sealer to root dentin.
MATERIALS AND METHODS: Seventy-two single-rooted human mandibular premolars were prepared using the rotary system to size 40 and randomly divided into four groups (n = 18) according to the final irrigation activation technique used as follows: conventional irrigation (CI), laser-activated irrigation with PIPS (LAI-PIPS), passive ultrasonic irrigation (PUI), and sonic irrigation (SI) with 5 mL of 17 % EDTA and 2.5 % NaOCl. The root canals were then obturated with gutta-percha and AH PlusJet sealer. A push-out test was used to measure the bond strength between the root canal dentin and the sealer. The data were analyzed using the two-way analysis of variance and least significant difference (LSD) post hoc tests (P = 0.05).
RESULTS: The LAI-PIPS and PUI resulted in higher push-out values compared to CI and SI (P < 0.05). There were no statistically significant differences between CI and SI (P = 0.978) and between LAI-PIPS and PUI (P = 0.051). There was a statistically significant interaction between the final irrigant activation techniques used and root canal thirds (P < 0.05). A chi-square test revealed no significant differences in the failure mode within the groups (P > 0.05).
CONCLUSIONS: The use of LAI-PIPS and PUI can provide higher bond strength of resin sealer to root dentin compared to CI and SI techniques.
CLINICAL RELEVANCE: The activation of the irrigant and the creation of the streaming have a positive effect on the bond strength of the resin sealer to root dentin.
PMID: 25315971 [PubMed - indexed for MEDLINE]