Cybermedlife - Therapeutic Actions IInfrared Light

Low infra red laser light irradiation on cultured neural cells: effects on mitochondria and cell viability after oxidative stress. 📎

Abstract Title: Low infra red laser light irradiation on cultured neural cells: effects on mitochondria and cell viability after oxidative stress. Abstract Source: BMC Complement Altern Med. 2009;9:8. Epub 2009 Apr 15. PMID: 19368718 Abstract Author(s): Alessandro Giuliani, Luca Lorenzini, Michele Gallamini, Alessandro Massella, Luciana Giardino, Laura Calzà Article Affiliation: BioPharmaNet-DIMORFIPA, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy. This email address is being protected from spambots. You need JavaScript enabled to view it. Abstract: BACKGROUND: Considerable interest has been aroused in recent years by the well-known notion that biological systems are sensitive to visible light. With clinical applications of visible radiation in the far-red to near-infrared region of the spectrum in mind, we explored the effect of coherent red light irradiation with extremely low energy transfer on a neural cell line derived from rat pheochromocytoma. We focused on the effect of pulsed light laser irradiation vis-à-vis two distinct biological effects: neurite elongation under NGF stimulus on laminin-collagen substrate and cell viability during oxidative stress. METHODS: We used a 670 nm laser, with extremely low peak power output (3 mW/cm2) and at an extremely low dose (0.45 mJ/cm2). Neurite elongation wasmeasured over three days in culture. The effect of coherent red light irradiation on cell reaction to oxidative stress was evaluated through live-recording of mitochondria membrane potential (MMP) using JC1 vital dye and laser-confocal microscopy, in the absence (photo bleaching) and in the presence(oxidative stress) of H2O2, and by means of the MTT cell viability assay. RESULTS: We found that laser irradiation stimulates NGF-induced neurite elongation on a laminin-collagen coated substrate and protects PC12 cells against oxidative stress. CONCLUSION: These data suggest that red light radiation protects the viability of cell culture in case of oxidative stress, as indicated by MMP measurement and MTT assay. It also stimulates neurite outgrowth, and this effect could also have positive implications for axonal protection. Article Published Date : Jan 01, 2009
Therapeutic Actions Infrared Light

NCBI pubmed

Cyanylated Cysteine Reports Site-Specific Changes at Protein-Protein Binding Interfaces Without Perturbation.

Cyanylated Cysteine Reports Site-Specific Changes at Protein-Protein Binding Interfaces Without Perturbation. Biochemistry. 2018 May 22;: Authors: Dalton SR, Vienneau AR, Burstein SR, Xu RJ, Linse S, Londergan CH Abstract To investigate the cyanylated cysteine vibrational probe group's ability to report on binding-induced changes along a protein-protein interface, the probe group was incorporated at several sites in a peptide of the calmodulin (CaM) binding domain of skeletal muscle myosin light chain kinase. Isothermal titration calorimetry was used to determine the binding thermodynamics between calmodulin and each peptide. For all probe positions, the binding affinity was nearly identical to that of the unlabeled peptide. The CN stretching infrared band was collected for each peptide free in solution and bound to calmodulin. Binding-induced shifts in the IR spectral frequencies were correlated with estimated solvent accessibility based on molecular dynamics simulations. This work generally suggests (1) that site-specific incorporation of this vibrational probe group does not cause major perturbations to its local structural environment, and (2) that this small probe group might be used quite broadly to map dynamic protein binding interfaces. However, site-specific perturbations due to artificial labeling groups can be somewhat unpredictable and should be evaluated on a site-by-site basis through complementary measurements. A fully quantitative, simulation-based interpretation of the rich probe IR spectra is still needed but appears to be possible given recent advances in simulation techniques. PMID: 29787228 [PubMed - as supplied by publisher]

Fault Detection Based on Near-Infrared Spectra for the Oil Desalting Process.

Fault Detection Based on Near-Infrared Spectra for the Oil Desalting Process. Appl Spectrosc. 2018 Jan 01;:3702818776022 Authors: Luan X, Jin M, Liu F Abstract The fault detection problem of the oil desalting process is investigated in this paper. Different from the traditional fault detection approaches based on measurable process variables, near-infrared (NIR) spectroscopy is applied to acquire the process fault information from the molecular vibrational signal. With the molecular spectra data, principal component analysis was explored to calculate the Hotelling T2 and squared prediction error, which act as fault indicators. Compared with the traditional fault detection approach based on measurable process variables, NIR spectra-based fault detection illustrates more sensitivity to early failure because of the fact that the changes in the molecular level can be identified earlier than the physical appearances on the process. The application results show that the detection time of the proposed method is earlier than the traditional method by about 200 min. PMID: 29786449 [PubMed - as supplied by publisher]

Suppression of pupillary unrest by general anesthesia and propofol sedation.

Related Articles Suppression of pupillary unrest by general anesthesia and propofol sedation. J Clin Monit Comput. 2018 May 21;: Authors: Behrends M, Larson MD, Neice AE, Bokoch MP Abstract The pupil undergoes irregular oscillations when exposed to light. These oscillations, known as pupillary unrest in ambient light, originate from oscillatory activity within the Edinger-Westphal nucleus in the midbrain. The midbrain and upper pons also contain nuclei known to be very sensitive to the effects of anesthetics that play a central role in maintaining wakefulness. We hypothesized that anesthetics may display similar effects on wakefulness and pupillary unrest. Repeat measurements of pupillary unrest using infrared pupillometry were performed in 16 patients undergoing general anesthesia and 8 patients undergoing propofol sedation. Pupil scans were analyzed using fast Fourier transformation to quantify the effects of the anesthetics on pupillary unrest. During general anesthesia and deep sedation, observed pupillary unrest values below 0.1 (AU) indicate complete suppression of pupillary oscillations. Pupillary unrest decreased more during general anesthesia [to 24% of baseline (95% CI 17-30%)] than pupil size [51% of baseline (95% CI 45-57%)]. Sedation with propofol was associated with a reduction in pupillary unrest that was correlated to the depth of sedation as assessed by the Richmond Agitation-Sedation Scale and the processed electroencephalogram. Pupillary unrest is caused by oscillatory activity within the midbrain that is affected by the state of wakefulness or by hypnotics directly. Increased sedation and general anesthesia reduce and then abolish pupillary unrest as wakefulness decreases. We speculate that midbrain nuclei responsible for wakefulness and pupillary unrest are either communicating or share a similar sensitivity to the effects of commonly used anesthetics. PMID: 29785552 [PubMed - as supplied by publisher]

An immunoconjugated up-conversion nanocomplex for selective imaging and photodynamic therapy against HER2-positive breast cancer.

Related Articles An immunoconjugated up-conversion nanocomplex for selective imaging and photodynamic therapy against HER2-positive breast cancer. Nanoscale. 2018 May 22;: Authors: Ramírez-García G, Panikar SS, López-Luke T, Piazza V, Honorato-Colin MA, Camacho-Villegas T, Hernández-Gutiérrez R, De la Rosa E Abstract Photodynamic therapy represents a very attractive therapeutic tool considered to be effective, minimally invasive and minimally toxic. However, conventional photodynamic therapy actually has two main constraints: the limited penetration depth of visible light needed for its activation, and the lack of selectivity. Considering this, this work reports the synthesis and evaluation of a novel nanoconjugate for imaging and selective photodynamic therapy against HER2-positive breast cancer, a particularly aggressive form of the disease. It was demonstrated that upon 975 nm near infrared light exposure, the red emission of the NaYF4:Yb,Er up-conversion nanoparticles (UCNPs) can be used for optical imaging and simultaneously represent the source for the excitation of a covalently bound zinc tetracarboxyphenoxy phthalocyanine (ZnPc), a photosensitizer that in turn transfers energy to ground state molecular oxygen to produce cytotoxic singlet oxygen. The specificity of our nanoconjugates was achieved by immunoconjugation with Trastuzumab (Tras), a specific monoclonal antibody for selective detection and treatment of HER2-overexpressing malignant breast cancer cells. Selective tracking of SKBR-3 HER2-positive cells was verified by confocal microscopy analysis, and the photodynamic therapy effect was considerably improved when Trastuzumab was incorporated into the nanoconjugate, the UCNPs-ZnPc-Tras being practically inert in the absence of infrared light exposure but reducing the HER2-positive cell viability up to 21% upon 5 min of the irradiation. This theranostic nanoconjugate represents a valuable alternative for HER2-positive breast cancer imaging and selective photodynamic therapy. PMID: 29785440 [PubMed - as supplied by publisher]

Near-infrared light-mediated photodynamic/photothermal therapy nanoplatform by the assembly of Fe3O4 carbon dots with graphitic black phosphorus quantum dots.

Related Articles Near-infrared light-mediated photodynamic/photothermal therapy nanoplatform by the assembly of Fe3O4 carbon dots with graphitic black phosphorus quantum dots. Int J Nanomedicine. 2018;13:2803-2819 Authors: Zhang M, Wang W, Cui Y, Zhou N, Shen J Abstract Background: Recently, combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become a desired treatment for cancer. However, the development of economic, high-efficiency, and safe photosensitizers/photothermal agents remains a significant challenge. Methods: A novel nanocomposite has been developed via the assembly of iron oxide carbon dot (Fe3O4-CDs) nanoparticles and black phosphorus quantum dots (genipin [GP]-polyglutamic acid [PGA]-Fe3O4-CDs@BPQDs), and this nanocomposite shows a broad light-absorption band and a photodegradable character. Results: In vitro and in vivo assays indicated that GP-PGA-Fe3O4-CDs@BPQDs were highly biocompatible and exhibited excellent tumor-inhibition efficacy, due to the synergistic PTT and PDT via a near-infrared laser. Importantly, in vivo tumor magnetic resonance imaging (MRI) results illustrated that GP-PGA-Fe3O4-CDs@BPQDs can be specifically applied for enhanced T2 MRI of tumors. This work presents the first combined application of a PDT and PTT effect deriving from BPQDs and MRI from Fe3O4-CDs, which may promote utilization of black BPQDs in biomedicine. Conclusion: As expected, GP-PGA-Fe3O4-CDs@BPQDs displayed a dramatically enhanced ability to destroy tumor cells, due to the synergistic combination of PTT and PDT. PMID: 29785107 [PubMed - in process]

Recent Progress in Upconversion Photodynamic Therapy.

Related Articles Recent Progress in Upconversion Photodynamic Therapy. Nanomaterials (Basel). 2018 May 18;8(5): Authors: Qiu H, Tan M, Ohulchanskyy TY, Lovell JF, Chen G Abstract Photodynamic therapy (PDT) is a minimally invasive cancer modality that combines a photosensitizer (PS), light, and oxygen. Introduction of new nanotechnologies holds potential to improve PDT performance. Upconversion nanoparticles (UCNPs) offer potentially advantageous benefits for PDT, attributed to their distinct photon upconverting feature. The ability to convert near-infrared (NIR) light into visible or even ultraviolet light via UCNPs allows for the activation of nearby PS agents to produce singlet oxygen, as most PS agents absorb visible and ultraviolet light. The use of a longer NIR wavelength permits light to penetrate deeper into tissue, and thus PDT of a deeper tissue can be effectively achieved with the incorporation of UCNPs. Recent progress in UCNP development has generated the possibility to employ a wide variety of NIR excitation sources in PDT. Use of UCNPs enables concurrent strategies for loading, targeting, and controlling the release of additional drugs. In this review article, recent progress in the development of UCNPs for PDT applications is summarized. PMID: 29783654 [PubMed]

Parameters sensitivity assessment and heat source localization using infrared imaging techniques.

Related Articles Parameters sensitivity assessment and heat source localization using infrared imaging techniques. Biomed Eng Online. 2017 Sep 21;16(1):113 Authors: Rastgar-Jazi M, Mohammadi F Abstract BACKGROUND: At present, infrared (IR) imaging is used both as a non-invasive and a non-ionizing technology. Using an IR camera, it is possible to measure body surface temperature in order to detect tumors and malignant cells. Tumors have a high amount of vasculature and an enhanced metabolism rate, which may result in an increase in body surface temperature by several degrees above its normal level. METHODS: Using thermograms, it is possible to assess various tumor parameters, such as depth, intensity, and radius. Also, by solving for Penne's bioheat equation, it is possible to develop the analytical method to solve for inverse heat conduction problem (IHCP). RESULTS: In the present study, these parameters were optimized using artificial neural networks in order to localize the heat source in the medium (i.e. female breast) more accurately. CONCLUSION: Eventually, a new formula was derived from Penne's bioheat equation to estimate the depth and radius of the embedded heat source. Moreover, by analyzing the data, errors of the parameters could be estimated. PMID: 28934956 [PubMed - indexed for MEDLINE]

Current treatments of acne: Medications, lights, lasers, and a novel 650-μs 1064-nm Nd: YAG laser.

Related Articles Current treatments of acne: Medications, lights, lasers, and a novel 650-μs 1064-nm Nd: YAG laser. J Cosmet Dermatol. 2017 Sep;16(3):303-318 Authors: Gold MH, Goldberg DJ, Nestor MS Abstract The treatment of acne, especially severe acne, remains a challenge to dermatologists. Therapies include retinoids, antibiotics, hormones, lights, lasers, and various combinations of these modalities. Acne is currently considered a chronic rather than an adolescent condition. The appropriate treatment depends on the patient and the severity of disease. The purpose of this study was to review current therapies for acne of all severities and to introduce the 650-μs 1064-nm laser for the treatment of acne. PMID: 28703382 [PubMed - indexed for MEDLINE]