Cybermedlife - Therapeutic Actions IInfrared Light

Low infra red laser light irradiation on cultured neural cells: effects on mitochondria and cell viability after oxidative stress. 📎

Abstract Title: Low infra red laser light irradiation on cultured neural cells: effects on mitochondria and cell viability after oxidative stress. Abstract Source: BMC Complement Altern Med. 2009;9:8. Epub 2009 Apr 15. PMID: 19368718 Abstract Author(s): Alessandro Giuliani, Luca Lorenzini, Michele Gallamini, Alessandro Massella, Luciana Giardino, Laura Calzà Article Affiliation: BioPharmaNet-DIMORFIPA, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy. This email address is being protected from spambots. You need JavaScript enabled to view it. Abstract: BACKGROUND: Considerable interest has been aroused in recent years by the well-known notion that biological systems are sensitive to visible light. With clinical applications of visible radiation in the far-red to near-infrared region of the spectrum in mind, we explored the effect of coherent red light irradiation with extremely low energy transfer on a neural cell line derived from rat pheochromocytoma. We focused on the effect of pulsed light laser irradiation vis-à-vis two distinct biological effects: neurite elongation under NGF stimulus on laminin-collagen substrate and cell viability during oxidative stress. METHODS: We used a 670 nm laser, with extremely low peak power output (3 mW/cm2) and at an extremely low dose (0.45 mJ/cm2). Neurite elongation wasmeasured over three days in culture. The effect of coherent red light irradiation on cell reaction to oxidative stress was evaluated through live-recording of mitochondria membrane potential (MMP) using JC1 vital dye and laser-confocal microscopy, in the absence (photo bleaching) and in the presence(oxidative stress) of H2O2, and by means of the MTT cell viability assay. RESULTS: We found that laser irradiation stimulates NGF-induced neurite elongation on a laminin-collagen coated substrate and protects PC12 cells against oxidative stress. CONCLUSION: These data suggest that red light radiation protects the viability of cell culture in case of oxidative stress, as indicated by MMP measurement and MTT assay. It also stimulates neurite outgrowth, and this effect could also have positive implications for axonal protection. Article Published Date : Jan 01, 2009
Therapeutic Actions Infrared Light

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CO2 selective PolyActiveTM membrane: Thermal transitions and gas permeance as a function of thickness.

Related Articles CO2 selective PolyActiveTM membrane: Thermal transitions and gas permeance as a function of thickness. ACS Appl Mater Interfaces. 2018 Jul 18;: Authors: Rahman MM, Abetz C, Shishatskiy S, Martin J, Müller AJ, Abetz V Abstract It is generally accepted that the melting point of a semicrystalline polymer is associated with the thickness of the crystalline lamellae (Gibbs-Thomson equation). In this study a commercially available multiblock copolymer PolyActive™ composed of 77 wt% of poly(ethylene glycol terephthalate) (PEGT) and 23 wt% of poly(butylene terephthalate) (PBT) was dip coated on top of a multilayer microporous support. The thickness was changed between 0.2 - 8 μm using coating solutions containing 0.75 - 7.5 wt% PolyActive™. The surface temperature of the membrane during dip coating was monitored using an infrared camera. Single gas permeances of N2, H2, CH4 and CO2 were measured between 20 ºC and 80 ºC at temperature steps of 2 ºC. Spherulitic superstructures composed of radially directed lamellae were observed in the polarized light microscope in all of the prepared membranes. Atomic force microscopy studies showed that the thickness of the crystalline lamellae was in the order of 10 nm or 0.01 µm at the surface of the membrane. Therefore according to the Gibbs-Thomson equation the melting point should not change in the thickness range 0.2 - 8 µm. But the gas permeance data showed that the melting point of the polyether domains of the 0.2 µm PolyActiveTM layer was 10 ºC lower compared to that of the 8 µm layer. The results can be explained by considering that the width of many crystalline lamellae significantly reduce as a function of film thickness, thereby reducing the average fold surface free energy/lateral surface free energy ratio. PMID: 30019890 [PubMed - as supplied by publisher]

Cyano-tryptophans as dual infrared and fluorescence spectroscopic labels to assess structural dynamics in proteins.

Related Articles Cyano-tryptophans as dual infrared and fluorescence spectroscopic labels to assess structural dynamics in proteins. Phys Chem Chem Phys. 2018 Jul 18;: Authors: van Wilderen LJGW, Brunst H, Gustmann H, Wachtveitl J, Broos J, Bredenbeck J Abstract The steady state and time-resolved fluorescence and infrared (IR) properties of 4- and 5-cyanotryptophan (CNTrp) are investigated and compared, and the tryptophan (Trp) analogs are found to be very attractive to study structural and dynamic properties of proteins. The position of the nitrile substitution as well as the solvent environment influences the spectroscopic properties (solvatochromism). Similar to native Trp, electronic (nanosecond) lifetime and emission spectra are modulated by the environment, making CNTrps attractive fluorescent probes to study the structural dynamics of proteins in complex media. The nitrile absorption in the IR region can provide local structural information as it responds sensitively to changes in electrostatics and hydrogen bond (HB) interactions. Importantly, we find that 4CNTrp exhibits a single absorption in the nitrile stretch region, while the model compound 4CN-indole (4CNI) shows two. Even though the spectrum of the model compound is perturbed by a Fermi resonance, we find that 4CNTrp itself is a useful IR label. Moreover, if the nitrile group is substituted at the 5 position, the Trp analog predominantly reports on its HB status. Because the current literature on similar compounds is too limited for a detailed solvatochromic analysis, we extend the available data significantly. Only now are microscopic details such as the mentioned sensitivity to electrostatics coming to light. The vibrational lifetime of the CN moiety (acting on a picosecond time scale in contrast to the nanosecond time scale for fluorescent emission) allows for its application in 2D-IR spectroscopy in the low picosecond range. Taken together, the benefits of CNTrps are that they absorb and emit separately from the naturally occurring Trp and that in these dual fluorescence/vibrational labels, observables of IR and fluorescence spectroscopy are modulated differently by their surroundings. Because IR absorption and fluorescence operate on different time and length scales, they thus provide complementary structural information. PMID: 30019716 [PubMed - as supplied by publisher]

Mesenteric ischemia in acute aortic dissection.

Related Articles Mesenteric ischemia in acute aortic dissection. Gen Thorac Cardiovasc Surg. 2018 Jul 17;: Authors: Orihashi K Abstract Despite improved outcomes in acute aortic dissection in Japan, mesenteric ischemia remains a problem. This is largely due to difficulties in early diagnosis and treatment. To address this problem, this article will review several diagnostic modalities such as ultrasound and near-infrared spectroscopy, as well as surgical treatments. Ultrasound is useful for assessing mesenteric ischemia because it can be performed at bedside and it provides information on both morphology and perfusion in real time without exposure to radiation or contrast media. Malperfusion can be assessed at four sites between the aorta and intestine. Two causes of malperfusion, aortic type and branch type, can be assessed. Lack of intestinal movement can also indicate ischemia. Near-infrared light assesses regional oxygen saturation in the tissue, while fluorescent visualization using indocyanin green demonstrates vessels and tissue perfusion. Information provided by these imaging modalities can help guide treatment strategies. Future treatments include endovascular interventions combined with conventional surgical procedures. The hybrid OR may be helpful for such sophisticated treatments. PMID: 30019252 [PubMed - as supplied by publisher]

Two-dimensional tellurium nanosheets for photoacoustic imaging-guided photodynamic therapy.

Related Articles Two-dimensional tellurium nanosheets for photoacoustic imaging-guided photodynamic therapy. Chem Commun (Camb). 2018 Jul 18;: Authors: Lin Y, Wu Y, Wang R, Tao G, Luo PF, Lin X, Huang G, Li J, Yang HH Abstract We report the synthesis of two-dimensional Te nanosheets through a facile liquid exfoliation method. The as-synthesized Te nanosheets can produce reactive oxygen species under light irradiation and show high photoacoustic imaging performance due to their strong near-infrared absorbance, and can be engineered as a nanoplatform for simultaneous photoacoustic imaging and photodynamic therapy. PMID: 30019046 [PubMed - as supplied by publisher]

Structural Study of Europium Doped Gadolinium Polyphosphates LiGd(PO3)4 and Its Effect on Their Spectroscopic, Thermal, Magnetic, and Optical Properties.

Related Articles Structural Study of Europium Doped Gadolinium Polyphosphates LiGd(PO3)4 and Its Effect on Their Spectroscopic, Thermal, Magnetic, and Optical Properties. Int J Anal Chem. 2018;2018:4371064 Authors: Hammami S, Boudjada NC, Megriche A Abstract Alkali metal-rare earth polyphosphates LiGd(1-x)Eux(PO3)4 (LGP:Eu3+) (where x= 0, 0.02 and 0.04) were synthesized by solid-state reaction. The Rietveld refinement showed the following cell parameters: I 2/a space group, a=9.635(3) Å, b=7.035(3) Å, c=13.191(3) Å, β=90.082°, V= 894.214Å3, and Z=4. The similarity between RF=4.21% and RB=4.31% indicated that the realized refinement is reliable. The crystal structure consists of infinite zig-zag chains of (PO4)3- tetrahedra, linked by bridging oxygen. The acyclic structure of polyphosphates is confirmed by infrared and Raman (IR) spectroscopies. A good thermal stability up to 940°C and paramagnetic behavior of these compounds were also proved by thermal analyses and magnetic susceptibility measurements, respectively. Excitation spectra revealed the charge transfer phenomenon between O2- and Eu3+ (CTB), the energy transfer from Gd3+ to Eu3+, and the intrinsic 4f-4f transitions of Eu3+ where the electronic transitions were also identified. Moreover, LGP:Eu3+ can emit intense reddish orange light under excitation at 394 nm. The strongest tow at 578 and 601 nm can be attributed to the transitions from excited state 5D0 to ground states 7F1 and 7F2, respectively. PMID: 30018642 [PubMed]

Purification and biochemical characterization of photo-active membrane protein bacteriorhodopsin from Haloarcula marismortui, an extreme halophile from the Dead Sea.

Related Articles Purification and biochemical characterization of photo-active membrane protein bacteriorhodopsin from Haloarcula marismortui, an extreme halophile from the Dead Sea. Int J Biol Macromol. 2018 Jul 12;: Authors: Alsafadi D, Khalili FI, Juwhari H, Lahlouh B Abstract Bacteriorhodopsin (BR) is an exciting photo-active retinal protein with many potential industrial applications. In this study, BR from the extremely halophilic archaeon Haloarcula marismortui (HmBR) was purified successfully using aqueous two phase extraction method. Absorption spectroscopy analysis showed maximum absorption peak of HmBR retinal protein (λmax) at 415 nm. The purified HmBR was visualized by SDS-PAGE, with a subunit molecular mass of 27 kDa, and its identity was confirmed by resonance Raman spectroscopy, Fourier transform infrared spectroscopy and atomic force microscopy. The effect of pH and salt concentration on the absorption spectrum of HmBR was evaluated. Red-shifted in λmax of HmBR was recorded at acidic condition (pH 5) and HmBR showed remarkable optical activity under high salinity condition. The photoelectric activity of HmBR was evaluated by measuring the DC-voltage generated from HmBR coated on indium tin oxide (ITO) glass when light illumination was applied. PMID: 30017983 [PubMed - as supplied by publisher]

Light-emitting diode therapy (photobiomodulation) effects on oxygen uptake and cardiac output dynamics during moderate exercise transitions: a randomized, crossover, double-blind, and placebo-controlled study.

Related Articles Light-emitting diode therapy (photobiomodulation) effects on oxygen uptake and cardiac output dynamics during moderate exercise transitions: a randomized, crossover, double-blind, and placebo-controlled study. Lasers Med Sci. 2018 Jul;33(5):1065-1071 Authors: Beltrame T, Ferraresi C, Parizotto NA, Bagnato VS, Hughson RL Abstract Light-emitting diodes (LEDs) might have a beneficial impact on cytochrome-c oxidase enzyme activity. Thus, it was hypothesized that photobiomodulation by light-emitting diode therapy (LEDT) could influence aerobic metabolism dynamics. Possible LEDT-mediated aerobic improvements were investigated mainly by a precise characterization of the pulmonary O2 uptake dynamics during moderate exercise transitions. Eight healthy young adults were enrolled in this randomized, double-blind, placebo-controlled, crossover study. A multi-diode array of LEDs was used for muscular pre-conditioning 30 min and 6 h before exercise testing. Pulmonary O2 uptake, carbon dioxide output, cardiac output, heart rate, stroke volume, and total arteriovenous oxygen difference dynamics were evaluated by frequency domain analysis. Comparisons revealed no statistical (p > 0.05) differences between LEDT and placebo, suggesting no significant changes in aerobic system dynamics. These results challenge earlier publications that reported changes in pulmonary O2 uptake during incremental exercise until exhaustion after LEDT. Perhaps, increments in peak pulmonary O2 uptake after LEDT may be a consequence of higher exercise tolerance caused by non-aerobic-related factors as opposed to an improved aerobic response. PMID: 29516305 [PubMed - indexed for MEDLINE]

Near infra red spectroscopy: a tool for solid state characterization.

Related Articles Near infra red spectroscopy: a tool for solid state characterization. Drug Discov Today. 2017 Dec;22(12):1835-1843 Authors: Chavan RB, Bhargavi N, Lodagekar A, Shastri NR Abstract Physical characterization of solid form of drug is of paramount importance as its biopharmaceutical properties and/or its processing behavior may be altered. Early identification and monitoring of solid state transformation is a critical requirement for pharmaceutical product development. In combination with chemometrics, a non destructive and non invasive technique like NIR is a powerful tool for solid state characterization. Main focus of this review is application of NIR for qualitative and quantitative analysis of solid forms of drugs and excipients. In addition, this review also sheds light on recent advancement in NIR, such as NIR chemical imaging and NIR based hyphenated techniques. PMID: 28893511 [PubMed - indexed for MEDLINE]