Actions of redox-active compound resveratrol under hydrogen peroxide insult in C6 astroglial cells.
Toxicol In Vitro. 2010 Apr;24(3):916-20. Epub 2009 Nov 27. PMID: 19945524
Research Group in Neuroglial Plasticity, Department of Biochemistry, Institute of Health's Basic Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. This email address is being protected from spambots. You need JavaScript enabled to view it.
The mechanisms by which resveratrol (3,5,4'-trihydroxy-stilbene) imparts neural effects is not well understood. We previously demonstrated that, depending upon the concentration of resveratrol and the cell type, this compound exerts anti-or pro-oxidant effects. In the present study, we investigated the effects of resveratrol on H(2)O(2)-mediated genotoxicity in C6 astroglial cells (I - 1mM H(2)O(2)/30 min or II - 0.1mM H(2)O(2)/6h), evaluated by micronucleus assay, lipid peroxidation (TBARS) and membrane integrity. H(2)O(2) increased micronuclei to 1.5 (I) and 1.7-fold (II), compared to control cells. This DNA damage was prevented (I) or partially prevented (II) by resveratrol. Oxidative insult also increased TBARS, 52% in I and 38% in II, P<0.05. These effects were prevented by resveratrol in I and increased in II (70% of increase). Present data contribute to the understanding of resveratrol effects under oxidative stress damage.
